Phytochemical Analysis And Potential Anticancer Activity Of Propolis Extract Derived From Malaysian Trigona Apicalis In Human Cervical Cells

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dc.contributor.authorAbdul Gapar, Nur Adilah
dc.date.accessioned2019-04-09T08:28:05Z
dc.date.available2019-04-09T08:28:05Z
dc.date.issued2018-04
dc.description.abstractPropolis is a resinous substance of beehive which is made from various sources of a plant. It exists in yellow to dark brown in colour depending on its age and sources. Propolis is well known for its therapeutic properties. It possesses a wide range of biological activities such as antioxidant and anticancer properties. The impact of different solvents of various degree of polarities, on the extraction of antioxidant compounds of Malaysian stingless bee propolis, Trigona apicalis, were studied. Ethanolic extract was found to have the highest yield (28.6 %). The efficiency of solvents for extraction of propolis was found to be in the order of Ethanol > Aqueous> Dichloromethane > Petroleum ether. The phenolic acid ranged from 32.45 to 85 μg gallic acid equivalents/g while the flavanoid range from 13 to 147.44 μg quercetin equivalents/g. Ethanolic extract of propolis had the highest phenolic content and was the most potent scavenger of DPPH and ABTS. Meanwhile, the aqueous extract had the highest FRAP value, which correlates with the highest flavanoid composition. A positive correlation existed in regards to the phenolic and flavanoid content with the antioxidant activities of Trigona apicalis propolis. In this study, both polar extracts were examined for the anticancer properties on HeLa cell line. The result obtained shows that ethanolic and aqueous propolis extracts exhibited potential anticancer agent with inhibition of 50% at 31.25 and 120 μg/mL respectively.The extracts killed the HeLa cells via apoptosis mechanism by killing the cells more than 50% for ethanolic extract and about 30% for aqueous extract at maximum dose concentration; 62.5 and 160 μg/mL, respectively. Beside when tested for cell cycle arrest, ethanolic extract caused cell arrest at G0/G1 phase. Meanwhile, aqueous propolis extract caused cell arrest at G2/M phase.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/7952
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectPotential anticancer activity of propolis extracten_US
dc.subjectTrigona apicalis in human cervical cellsen_US
dc.titlePhytochemical Analysis And Potential Anticancer Activity Of Propolis Extract Derived From Malaysian Trigona Apicalis In Human Cervical Cellsen_US
dc.typeThesisen_US
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