The Study Of Semiochemicals And Their Application In The Control Of Aedes Aegypti (Linnaeus)
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Date
2013-12
Authors
Ong, Song Quan
Journal Title
Journal ISSN
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Publisher
Universiti Sains Malaysia
Abstract
In the present study, the attract-and-kill strategy was demonstrated in the larva and adult of Aedes aegypti. The two components in the strategy: attractant and killing agent were determined and bioassay of the combination to Ae. aegypti was carried out. In the larval stage, extraction of the Ae. aegypti oviposition pheromone was performed using fatty acid extraction and modified methanolic extraction from the eggs of Ae. Aegypti (lab strain) from Vector Control Research Unit (VCRU), University Sains Malaysia. Fourteen compounds were identified by fatty acid while eight compounds were identified by modified methanolic extraction. Among these, four compounds (palmitoleic acid, caproic acid, palmitic acid and fumaric acid) were investigated for their attractiveness to gravid female Ae. aegypti; 1ppm caproic acid significantly attracted more gravid female Ae. aegypti to lay eggs (660.83±91.61) than the control (343.83±56.24). Combination of caproic acid with granules temephos as a lethal lure has significantly attracted more gravid female Ae. aegypti to oviposite their eggs (835.40 ± 94.23, P>0.001) than the control (538.30 ± 74.50, P>0.001) and the presence of temephos restricted the hatching of the eggs and kill the progeny when the larvae hatched. Meanwhile, host-seeking behaviour of female Ae. aegypti is influenced by various cues in which odours play major part in this behaviour. The attractiveness of several kairomones: L-lactic acid, ammonia, acetone and acetic acid were tested in its individual and binary combinations. Ammonia-acetone and ammonia-acetic acid significantly attracted more female Ae.
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Keywords
Semiochemicals , Aedes Aegypti (Linnaeus)