Formation Of Stable Gold Nanoparticles Via Laser Ablation Conjugated In-Situ By BSA In Harsh Saline Condition For Cells Antiproliferative Activity
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Date
2018-03
Authors
Abdulateef, Sinan Adnan
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Colloidal AuNPs is known to aggregate and precipitate in the saline solution
below the NaCl concentration of many bodily fluids and blood plasma. This research
focused on the preparation of high stability and bioactivity colloidal gold nanoparticles
(AuNPs) in simulated body fluid (SBF), which can closely mimic the blood plasma
that is free from contamination through a low-cost technique that can be done at room
temperature within a short period of time. To achieve this aim, the following three
specific objectives were designed; Firstly, to produce a high stability colloidal
suspension of AuNPs in media close to blood plasma by using pulse laser ablation
synthesis techniques. Secondly, to use in situ generation of the colloidal AuNPs -
bovine serum albumin bioconjugated system for pulsed laser ablation. Here, bovine
serum albumin is adsorbed to the AuNP surface to enhance the stability and to improve
its biocompatibility. Thirdly, to evaluate the anti-cancer activity towards human
cervical cancer cell lines (HeLa). Q – Switch laser pulse of 6 nanoseconds was
employed to produce a colloidal suspension of AuNPs by laser ablation.
Comprehensive, systematical characterization of the samples, for their structural,
morphological (electron diffraction, high-resolution transmittance electron
microscopy, optical properties (UV-Vis absorption, light dynamic scattering, and
Fourier transform infrared spectroscopy) and electrical (zeta potential) properties,
have been carried out. The laser ablation approach provides controllable process size,
size distribution, and shape of NPs. Effects of laser fluence have been examined on the
ablation efficiency, which is useful in concentration and productivity.
Description
Keywords
Formation of stable gold nanoparticles via laser ablation , conjugated in-situ by BSA in harsh saline condition