Formation Of Stable Gold Nanoparticles Via Laser Ablation Conjugated In-Situ By BSA In Harsh Saline Condition For Cells Antiproliferative Activity

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dc.contributor.authorAbdulateef, Sinan Adnan
dc.date.accessioned2019-05-02T07:11:00Z
dc.date.available2019-05-02T07:11:00Z
dc.date.issued2018-03
dc.description.abstractColloidal AuNPs is known to aggregate and precipitate in the saline solution below the NaCl concentration of many bodily fluids and blood plasma. This research focused on the preparation of high stability and bioactivity colloidal gold nanoparticles (AuNPs) in simulated body fluid (SBF), which can closely mimic the blood plasma that is free from contamination through a low-cost technique that can be done at room temperature within a short period of time. To achieve this aim, the following three specific objectives were designed; Firstly, to produce a high stability colloidal suspension of AuNPs in media close to blood plasma by using pulse laser ablation synthesis techniques. Secondly, to use in situ generation of the colloidal AuNPs - bovine serum albumin bioconjugated system for pulsed laser ablation. Here, bovine serum albumin is adsorbed to the AuNP surface to enhance the stability and to improve its biocompatibility. Thirdly, to evaluate the anti-cancer activity towards human cervical cancer cell lines (HeLa). Q – Switch laser pulse of 6 nanoseconds was employed to produce a colloidal suspension of AuNPs by laser ablation. Comprehensive, systematical characterization of the samples, for their structural, morphological (electron diffraction, high-resolution transmittance electron microscopy, optical properties (UV-Vis absorption, light dynamic scattering, and Fourier transform infrared spectroscopy) and electrical (zeta potential) properties, have been carried out. The laser ablation approach provides controllable process size, size distribution, and shape of NPs. Effects of laser fluence have been examined on the ablation efficiency, which is useful in concentration and productivity.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8135
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectFormation of stable gold nanoparticles via laser ablationen_US
dc.subjectconjugated in-situ by BSA in harsh saline conditionen_US
dc.titleFormation Of Stable Gold Nanoparticles Via Laser Ablation Conjugated In-Situ By BSA In Harsh Saline Condition For Cells Antiproliferative Activityen_US
dc.typeThesisen_US
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