Production of tannase by aspergillus niger using mangrove (rhizophora apiculata) bark under solid substrate fermentation.
| dc.contributor.author | Wee Yee, Tan | |
| dc.date.accessioned | 2016-01-13T02:08:38Z | |
| dc.date.available | 2016-01-13T02:08:38Z | |
| dc.date.issued | 2010-08 | |
| dc.description.abstract | Rhizophora apiculata bark is tannin rich waste material from charcoal industry, and was utilized as the solid substrate for the production of tannase in solid substrate fermentation system. Upon improvement of physical and chemical parameters via a simple shake flask system, combination of0.75 mm and 2.00 mm of substrate particles, Czapek-Dox medium (pH7) as moisturizing agent, initial moisture content of 1:1.5 (w/v), incubation temperature at 30° C, mixing at every 24 hours and inoculum size of 1 x 106 spores/ml showedtannaseachieved4.97 U/g of fermented substrate on the 61 h day of cultivation time. After supplementation of 5% (w/w) tannic acid and 1% (w/w) of urea, tannase achieved 39.34 U/g of fermented substrate after 6 days of cultivation. Enzyme activity increased 1280.35% compared to initial profile (2.85 U/g of fermented substrate). A tray system (15.0 em x 15.0 em x 7.5 em) was carried out to scale-up the production of enzyme. After improvement of several key process parameters, substrate bed height of 0.6 em (50 g of solid substrates), moisture content at 1:1.5 (w/v), mixing at every 48 hours during the cultivation period, inoculum size of 1 x l 06 spores/ml and 6 days of cultivation time showed that tannase was 63. 24 U/g of fermented substrate. A further scale-up with 1 XXV kg of solid substrates via a bigger tray system (32 em x 22 em x II em) showed that tannase production achieved 127. 13 U/g of fermented substrate on the day 14 of cultivation. Enzyme activity increased 4360.70 % compared to initial profile (2.85U/g) from shake flask system. Crude enzyme was concentrated and purifiedabout 55 fold with specific activity of 1.1 0, recovery at 0.02 % and molecular weight of 55.92 kDa. Optimum reaction temperature for both crude and purified enzymes was 70°C and stable at the range of 30-70°C around 1 hour. Optimum pH for crude and purified enzyme was pH 6 and pH 5.5, respectively. They were stable at pH 5.0 and 5.5, respectively, around 1 hour. Tannic acid at 0.4% (w/v) was specific substrate for both crude and purified tannase. Metal ions of At3+, Ba2+, Ca2+, Co2 +, Cu2+, Fe2+, K\ Li2+, Mg2+, Mn2+, Na+ an~ Zn2+ inhibited the crude and purified tannase activities. Substrate degradation evaluation with the aid of light microscope, SEM and TEM showed the effect ofbiodegradation by Aspergillus niger and activity of tannase on fermented substrate. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/1557 | |
| dc.language.iso | en | en_US |
| dc.subject | Aspergillus niger | en_US |
| dc.subject | (Rhizophora apiculata) | en_US |
| dc.subject | Solid substrate fermentation. | en_US |
| dc.title | Production of tannase by aspergillus niger using mangrove (rhizophora apiculata) bark under solid substrate fermentation. | en_US |
| dc.type | Thesis | en_US |
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