Development Of Poly(Lactic Acid) Microspheres/Gelatin Coated Beta-Tricalcium Phosphate Scaffold For Bone Tissue Engineering Application
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Date
2019-04-01
Authors
Cheng, Shu Yen
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Three-dimensional interconnected porous structure and ideal mechanical
strength are essential for biological scaffold in bone tissue engineering application.
The main aim of this study is to develop multifunctional scaffold for bone tissue
engineering application. In this research, gel casting method was used to produce
porous beta-tricalcium phosphate (β-TCP) scaffold. β-TCP scaffold coated with
gelatin crosslinked with 1% glutaraldehyde (GA) showed optimum compressive
strength where it improved around 139% compressive strength compared to uncoated
scaffold. Uncoated β-TCP scaffold and the β-TCP scaffold coated with crosslinked
gelatin showed their bioactive properties by taking 2 weeks to form apatite. The
gelatin coating had slightly reduced protein-adsorption compared to pure β-TCP. In
the second part, emulsion solvent evaporation was used to fabricate doxycycline
loaded poly(lactic acid) (PLA) microspheres. Results showed that the 4% NaCl in
external aqueous phase was the most efficient way to increase the encapsulation
efficiency where 66% of the doxycycline was encapsulated and showed the largest
inhibition growth zone diameter of E. coli at 16.15mm. Lastly, β-TCP scaffold
coated with PLA microspheres had enhanced the compressive strength. The
encapsulated doxycycline was released in a slower and sustained manner compared
to free PLA microspheres. In short, PLA microspheres coated scaffold developed in
the study shows multifunctional as it provides structural support, bioactivity and
controlled drug delivery function for bone tissue engineering.