Cytotoxicity testing of chitosan derivatives on primary cultured fibroblast

dc.contributor.author	Nurul Firdaus Abdul Hadi
dc.date.accessioned	2021-03-10T08:35:55Z
dc.date.available	2021-03-10T08:35:55Z
dc.date.issued	2006-03
dc.description.abstract	Cbitosan has been used in several studies as wound healing accelerator. This study was designed to determine the cytotoxicity of cbitosan on primary cultured human fibroblasts. Human skins were obtained from surgical specimen of consented donor and primary cultured fibroblasts were established. The fibroblasts were incubated in the absence or presence of increasing concentrations of Oligo Chito 1% and Oligo Chito 5% for 24, 48 and 72 hours. Subsequently, MIT reagent was added and 4 hours later the purple crystals produced were dissolved in DMSO. Then, cells viability was read using ELISA plate reader. Cells viability was used as a marker for toxicity. The results showed that cell viability was dependent on concentration. The cell viability decreased when high concentrations of chitosan were used. Prolonged incubation period also caused decreased cell viability. Analysis of chitosan effects on cell culture is useful as a screening tool for their potential in vivo activity as wound healing agents.	en_US
dc.identifier.uri	http://hdl.handle.net/123456789/11976
dc.language.iso	en	en_US
dc.publisher	Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia	en_US
dc.subject	Healing accelerator	en_US
dc.title	Cytotoxicity testing of chitosan derivatives on primary cultured fibroblast	en_US
dc.type	Other	en_US

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