Expression Of Recombinant Human Dna Topoisomerase In Pichia Pastoris And Analysis Of Its Activity

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dc.contributor.authorChan, Mooi Kwai
dc.date.accessioned2020-10-22T01:33:54Z
dc.date.available2020-10-22T01:33:54Z
dc.date.issued2017-11
dc.description.abstractThis study was aimed to establish a method to produce in-house recombinant human DNA topoisomerase. The DNA topoisomerase I (Topo I) and DNA topoisomerase II (Topo II) coding regions were amplified from the cDNA of breast cancer cells, MDA-MB-231. Topo I and Topo II coding region were ligated into expression vector, pPICZ--A and pPICZ--C, respectively. Subsequently, the constructed recombinant vectors were transformed into four types of Pichia pastoris strain, X-33, GS115, SMD1168H and KM71H. PCR screening for recombinant clones was performed and followed by selection of multicopy clones using agar plates containing increasing concentrations of Zeocin.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/10539
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectRecombinant Human Dna Topoisomerase In Pichia Pastorisen_US
dc.subjectTopoisomerase In Pichia Pastoris And Analysis Of Its Activityen_US
dc.titleExpression Of Recombinant Human Dna Topoisomerase In Pichia Pastoris And Analysis Of Its Activityen_US
dc.typeThesisen_US
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