Assessment Of The Proliferation Rate Of Human Dental Pulp Stem Cells From Permanent And Deciduous Teeth Loaded On Granumas Scaffold
Loading...
Date
2011-02
Authors
Nasir, Naseer A.
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Many different human dental stem/progenitor cells have been isolated and
characterized such as dental pulp stem cells (DPSCs), stem cells from extracted
deciduous teeth (SHED), periodontal ligament stem cells (PDLSCs), stem cells from
apical papilla (SCAP), and dental follicle progenitor cells (DFPCs). These post-natal
populations have mesenchymal-stem-cell-like (MSCs) qualities, including the
capacity for self-renewal and multilineage differentiation potential. The aims of this
study were to isolate and characterize both DPSCs and SHED and to determine the
expression level of PCNA and Ki-67 by using immunocytochemistry and flow
cytometery. And to compare the proliferation rate of DPSCs and SHED on
GranuMas® and without GranuMas® scaffold, by using Alamar Blue® assay. Four
permanent teeth from four individuals aged 16 to 22 years old and four deciduous
teeth from four individuals aged 6 to 12 years old were obtained with informed
consent during tooth extraction procedure based on selected criteria and under
standardized procedures. According to the standard protocol the pulp then was
separated from the crown then digested in a solution of 3 mg/ml collagenase type I
and 4 mg/ml dispase for 1 hour at 37°C. Single-cell suspensions were obtained by
passing the cells through a 70-μm strainer (Falcon) and cultured in alpha
modification of Eagle's medium supplemented with 20% fetal bovine serum, 100 μM
L-ascorbic acid 2-phosphate, 2 mM L-glutamine, 100 units/ml penicillin and100
μg/ml streptomycin. The cells were incubated at 37°C in 5% CO2 and observed daily
under microscope.
Description
Keywords
Proliferation rate of human dental pulp stem cells , deciduous teeth loaded on Granumas scaffold