Screening Of Malaysian Patients With Nasopharyngeal Carcinoma (Npc) Using Comparative Genomic Hybridization (Cgh) Technique For Identification Of Specific Genetic Alteration
| dc.contributor.author | Muhamad Nor, Natasya Naili | |
| dc.date.accessioned | 2018-07-13T07:51:00Z | |
| dc.date.available | 2018-07-13T07:51:00Z | |
| dc.date.issued | 2012-03 | |
| dc.description.abstract | Nasopharyngeal Carcinoma (NPC) is a malignancy that originates from the epithelial lining of Fossa of Rosen Muller in the nasopharynx. It is a highly prevalent disease in Southern China and Southeast Asia including Malaysia. In this study, the genetic alterations from forty-four NPC patients from five hospitals in Malaysia were screened. Comparative Genomic Hybridization (CGH) technique was used for screening the genetic alterations. CGH is a molecular cytogenetic technique which is used to identify imbalanced genetic alterations that is present in this malignant disease. NPC tissues that were biopsied were first confirmed to have at least 75% tumour cells using Haematoxylin & Eosin (H & E) staining before proceeding to CGH. Tumor cells were extracted from forty-four NPC biopsies while forty-four references DNA were extracted from peripheral blood of forty-four normal controls. Tumour DNA and normal DNA was labelled by nick translation method with green and red fluorescent dyes. They were hybridized at metaphase chromosomes DNA and counterstained with DAPI II. Finally, the image was captured and analyzed. Most of the samples were male compared to female with a ratio of 2.4 : 1. Malay ethnic group was higher than Chinese which is 57% and 43% respectively. Chinese cases showed higher incidence in the two hospitals from the states with majority Chinese ethnic group. The mean age of diagnosis of the NPC patients is 49.45 years old (14.74) [mean (SD)]. There were no significant difference between age with gender and ethnicity. Most of the patients (38 patients) were NPC WHO Type III except 5 patients who were Type II and one Type I. There is no significant difference between WHO type with ethnic group and with gender of NPC patients. There is also no correlation between types of NPC with mean age of diagnosis. In this study, chromosomal alterations were found in 41 cases but none in 3 other cases. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/5923 | |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Sains Malaysia | en_US |
| dc.subject | Screening of malaysian patients with nasopharyngeal carcinoma | en_US |
| dc.subject | for identification of specific genetic alteration | en_US |
| dc.title | Screening Of Malaysian Patients With Nasopharyngeal Carcinoma (Npc) Using Comparative Genomic Hybridization (Cgh) Technique For Identification Of Specific Genetic Alteration | en_US |
| dc.type | Thesis | en_US |
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