A Study Of Burkholderia Pseudomallei K96243 Hypothetical Bpsl3393 Gene Product As A Putative Coa-Binding Protein
Loading...
Date
2014-01
Authors
Ooi, Gim Luan
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Burkholderia pseudomallei is the causative agent of melioidosis. From the whole
genomic comparison by using B. pseudomallei K96243 as reference genome, a total
48 genes were found specifically and common in B. pseudomallei strains. Amongst,
the hypothetical gene, BPSL3393, was deduced to contain the CoA-binding motif
based on its sequence analysis. However, this gene is still remaining as a putative
gene where it is still functionally uncharacterized. The objective of this study is to
elucidate the biological function of gene BPSL3393 in B. pseudomallei K96243. An
unmarked deletion mutant of gene BPSL3393 was constructed by using pDM4
suicidal vector. This vector employed catR, chloramphenicol resistant gene as the
selection marker and sacB, levansucrase gene as the counter-selection marker. The
biochemical profiles of ΔBPSL3393 mutant and wild type strains were determined
by using th Biolog GN2 MicroPlateTM system. The ΔBPSL3393 mutant showed
significant reduction in 2-aminoethanol utilization as compared to wild type. Apart
from this, the ΔBPSL3393 mutant also has shown some physiological difference as
compared to wild type. The ΔBPSL3393 mutant aggregated at the exponential phase
in M9 minimal media whereas the wild type did not. Thus, further study is needed to
characterize this ΔBPSL3393 mutant and to uncover its biological role.
Description
Keywords
Burkholderia Pseudomallei , Putative Coa-Binding Protein