The Effects And Mechanism Of Action Of Garcinia Atroviridis Extracts On The Lipid Profile And Antioxidant Status Of Chemically- And High Fat Diet-Induced Hyperlipidemic Rats
| dc.contributor.author | Al-Mansoub, Majed Ahmed Kacem | |
| dc.date.accessioned | 2018-01-18T02:44:20Z | |
| dc.date.available | 2018-01-18T02:44:20Z | |
| dc.date.issued | 2017-08 | |
| dc.description.abstract | Garcinia atroviridis, a member of Clusiaceae family, has gained much attention in recent years due to its ability to reduce body weight. The objective of this study was to investigate the effect and mechanism of action of G. atroviridis extracts on the lipid profile and antioxidant status of chemically- and high fat diet (HFD)- induced hyperlipidemic rats. Aqueous and methanolic extracts of different parts of G. atroviridis namely: ripe fruit with seeds, ripe fruit rind, unripe fruit with seeds, unripe fruit rind, leaves, and stem were used in this study. Generally, in the poloxamer- 407 (P-407)-induced acute hyperlipidemic rat model, the aqueous extracts showed better antihyperlipidemic activity than the methanol extracts. Of all the extracts, the aqueous extract of ripe fruit with seeds (AqE-RFS) showed the most potent antihyperlipidemic effect, comparable to that of atorvastatin (60 mg/kg). The AqERFS significantly decreased serum TC, TG, LDL and VLDL levels and AC at dose of 1000 mg/kg compared to that of hyperlipidemic control group at 58 hr after hyperlipidemia induction. Rats fed on HFD had significantly higher levels of serum lipid profile and elevation of serum and liver MDA associated with a significant decrease in GSH content and antioxidant enzymes activities. Daily treatment of HFD rats with AqE-RFS at 1000 and 500 mg/kg for six weeks showed significant reductions in TC, TG, and VLDL as compared to the HFD control rats. The AqE-RFS at 1000 mg/kg showed significant reduction in LDL. Likewise, the AC was also significantly reduced after treatment with 1000 and 500 mg/kg compared to HFD control group. Similarly, AqE-RFS treatment (1000 mg/kg) for six weeks significantly reduced lipid peroxidation as shown by decreased MDA level and increased GSH content as well as improvement in antioxidant activities of SOD, CAT, GPx and GR in liver and serum against oxidative damage in HFD rats. In contrast, for in vitro antioxidant screening, methanol extracts of the stem and leaves showed higher antioxidant activities than the fruits. A reversed phase (RP)-HPLC analysis for determination of two marker compounds, HCA and HCAL in all G. atroviridis extracts revealed that aqueous and methanol extracts of both unripe and ripe fruit rind contain the highest total contents of these markers, respectively. Elucidating of possible mechanism of action revealed that the AqE-RFS exhibited significant reduction on the activity of HMG-CoA reductase and moderate effect on pancreatic lipase. On the synthesis and excretion of lipids, the AqE-RFS significantly reduced the contents of liver TC and TG, as well as significantly increased fecal TC and bile acids excretion in a dose dependent manner. Safety assessment showed that AqE-RFS is non-toxic up to the dose of 2000 mg/kg for acute administration. Meanwhile, rats given chronic oral doses of AqE-RFS (250, 500 or 1000 mg/kg) survived the 3 months of dosing and showed no significant changes in general behaviour, hematological and biochemical parameters as well as histopathological observations of liver and kidney structures. In conclusion, these findings demonstrate the beneficial effect of AqE-RFS against hyperlipidemia and may be potential for development as antihyperlipidemic phytopharmaceuticals. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/5411 | |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Sains Malaysia | en_US |
| dc.subject | The effect and mechanism of action of G. atroviridis extracts | en_US |
| dc.subject | on the lipid profile and high fat diet | en_US |
| dc.title | The Effects And Mechanism Of Action Of Garcinia Atroviridis Extracts On The Lipid Profile And Antioxidant Status Of Chemically- And High Fat Diet-Induced Hyperlipidemic Rats | en_US |
| dc.type | Thesis | en_US |
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