Isolation, genome sequencing, assembly, annotation and Characterization of Thermus sp. CCB_US3_UF1 from Ulu Slim, Perak, Malaysia.
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Date
2011-02
Authors
Beng Soon, Teh
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Publisher
Universiti Sains Malaysia
Abstract
Thermus sp. CCB_US3_UF1, satu bacteria thermofilik telah Berjaya dipencilkan dari kolam air panas di Ulu Slim, Perak, Malaysia. Genomnya mengandungi 2,243,772 pasangan bes dan satu plasmid bersaiz 19,716 pasangan bes. Terdapat sebanyak 2293 jujukan pengkodan (rangka bacaan terbuka), 2 rRNA operon, 13 gen transposase dan 48 gen tRNA untuk semua 20 asid amino dalam genom ini. Annotasi genom meramalkan bahawa kitar asid trikarboksilik adalah lengkap. Semua protein/enzim dalam laluan metabolisme karbohidrat hadir.
Thermus sp. CCB_US3_UF1, a thermophilic bacterium has been successfully isolated from a hot spring in Ulu Slim, Perak, Malaysia. The genome consists of 2,243,772 bp and a 19,716 bp plasmid. There are 2293 predicted coding sequences (ORFs), 2 rRNA operons, 13 transposase genes, and 48 tRNA genes for all 20 amino acids in the genome. The genome annotation predicts that the tricarboxylic acid (TCA) cycle is complete. All the proteins/enzymes for carbohydrate metabolism pathways are present.
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Keywords
Isolation , Genome