Construction of baculovirus transfer vectors for insect cell expression of human choline and ethanolamine kinase isoforms

dc.contributor.author	Chang Chia, Than
dc.date.accessioned	2021-04-18T06:12:17Z
dc.date.available	2021-04-18T06:12:17Z
dc.date.issued	2008-10
dc.description.abstract	Phosphatidylcholine and phosphatidylethanolamine are the most abundant phospholipid in eukaryotic cell membrane. Hwnan choline kinase and ethanolamine kinase play a very important role in the biosynthesis of phosphatidylcholine and phosphatidylethanolamine by catalyzing in the first phosphorylation step of the pathway. In this study, genes encoding hwnan choline kinase-P (hCK-P) and ethanolamine kinase-2P (hEK-2P) isoform gene fragments were inserted into newly constructed plasmid. The plasmid was constructed from commercially purchased transfer plasmid pBAC4x-1. Open reading frames (ORF) ofhCK-P and hEK-2P were introduced into the downstream of polyhedrin promoter for expression as histidine-tagged (his-tag) proteins. The constructs were ready for homologous recombination with Autographa californica Nuclear Polyhedrosis Virus (AcNPV) genome. Insect cells infected with the recombinant AcNPV were expected to overexpress the hCK-P and hEK-2P proteins.	en_US
dc.identifier.uri	http://hdl.handle.net/123456789/12917
dc.language.iso	en	en_US
dc.publisher	Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia.	en_US
dc.subject	Eukaryotic cell	en_US
dc.title	Construction of baculovirus transfer vectors for insect cell expression of human choline and ethanolamine kinase isoforms	en_US
dc.type	Other	en_US

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