A comparative study on the protective effects of ascorbic acid and tualang honey on the skin flap of reverse sural fasciocutaneous flap in a smoking rabbit model
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Date
2015
Authors
Mohd Hussain, Mohd Shakir Bathusna
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
Abstract
Lower limb traumas impose a great challenge for reconstructive surgeons. Complexity
in the tissue constituent of lower limb and the concern regarding vascularity has been
the barrier in the process of reconstruction. Introduction of pedicled fasciocutaneous
flap based on reverse flow has provided an alternative to free flap reconstruction.
Reverse sural fasciocutaneous flap is gaining popularity and widely being used in the
reconstruction of distal third of lower limb due to its ease of elevation compared to free
flap.
Performing flap surgery in smokers has been proven to yield poor outcome.
Components in cigarette smoke have an established negative impact on the vascular
endothelium of blood vessels. Nicotine and carbon monoxide in cigarette smoke
produce oxidative damage and release reactive oxygen species resulting in ischaemic
injury.
Ascorbic acid and “Tualang honey” are two agents which have been extensively studied
for their anti oxidative and wound healing properties. Previous studies were aimed at
studying the impact of cigarette smoke and ascorbic acid on the endothelium of large
calibre vessel like carotid and aorta. The present study was undertaken to compare the
anti oxidative properties of both these agents in improving the outcome in flap survival
using a smoking rabbit model. This study is aimed at assessing the beneficial anti
oxidative property to improve microvascular circulation in a reverse sural
fasciocutaneous flap in a smoking rabbit model.
All samples were exposed to cigarette smoke using a smoke chamber for a total of 5
weeks. Study sample was divided into three groups. Control group only received
cigarette smoke while the other two groups each received ascorbic acid in the form of
intramuscular injection and oral “Tualang honey” on top of exposure to cigarette smoke.
Reverse sural fasciocutaneous flap was raised in all the rabbits at day 30 of exposure to
cigarette smoke.
Samples were evaluated on immediate post operative period, post operative day 1, 3 and
7. Parameters documented throughout the study were temperature, capillary refill time,
colour of flap, presence hematoma or infection, and on post operative day 7 the flap
survival was calculated. Intervention with smoking, ascorbic acid and “Tualang honey”
were continued throughout the period of experiment.
We found that, both group intervened with ascorbic acid and “Tualang honey” showed
marked improvement in the flap survival. Statistically significant improvement were
documented with regard to temperature, improvement in colour of flap and reduction in
hematoma. Flap survival improved from 62% in the smoking arm to 88.9% and 81.0%
in the ascorbic acid and “Tualang honey” arm. However oneway ANOVA test showed
p value of 0.075 which was near to significant value.
In conclusion both ascorbic acid and “Tualang honey” improved the survival of reverse
sural fasciocutaneous flap in smoking rabbit model. However ascorbic acid is superior
compared to “Tualang honey” in improving the oxidative damages produced by
cigarette smoke and microvascular circulation of the reverse sural fasciocutaneous flap.
Description
Keywords
Surgery, Plastic