Micropropagation Of Curcuma Xanthorrhiza Roxb. And Zingiber Aromaticum Vahl. With Antimicrobial Activities Via Aerated Culture System

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dc.contributor.authorKusumastuti, Melati Yulia
dc.date.accessioned2019-06-25T01:06:32Z
dc.date.available2019-06-25T01:06:32Z
dc.date.issued2012
dc.description.abstractThe aseptic rhizomatous buds of Curcuma xanthorrhiza and Zingiber aromaticum were established by surface sterilizing with 70% ethanol for 20 minutes followed by 50% (v/v) Clorox® for 20 minutes. This procedure produced 100% and 83.3% aseptic buds explants of C. xamthorrhiza and Z. aromaticum respectively. All the aseptic buds of both species survived when cultured on MS medium supplemented with 0.5 mg/L BA and 0.5 mg/L IBA. The number of shoots formed from each shoot explant for both species using MS medium supplemented with 1.0 mg/L BA (C. xanthorrhiza 3..7 shoots per explant; Z. aromaticum 4.3 shoots per explant) was found to be not significantly different when compared with cultures using MS supplemented 0.5 mg/L BA and 0.5 mg/L IBA (C. xanthorrhiza 3.9 shoots per explant; Z. aromaticum 3.0 shoots per explant). Hence MS medium supplemented with 0.5 mg/L BA and 0.5 mg/L IBA as the shoot proliferation medium was used for both species understudied. However, longitudinally cut shoots (half shoots) explant of C. xanthorrhiza and Z. aromaticum produced an average of 6.3 and 2.9 shoot per explant respectively (equivalent 12.6 and 6.8 shoot per whole shoot) using the shoot proliferation medium. The number of shoot produced from each half shoot explants was significantly higher than the whole shoot explants. A total of 524 and 329 shoots could be produced respectively from the C. xanthorrhiza and Z. aromaticum 200 half-shoot explants (equivalent to 100 whole shoots). Shoot explants of C. xanthorrhiza and Z. aromaticum cultured on liquid proliferation medium produced more multiple shoots than the agar-gelled medium.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8360
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectMicropropagation of Curcuma xanthorrhiza Roxben_US
dc.subjectantimicrobial activities via aerated culture systemen_US
dc.titleMicropropagation Of Curcuma Xanthorrhiza Roxb. And Zingiber Aromaticum Vahl. With Antimicrobial Activities Via Aerated Culture Systemen_US
dc.typeThesisen_US
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