Identification And Mode Of Symbiosis For Mycorrhizal Fungi And Epiphytic Orchid, Dendrobium Crumenatum
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Date
2016-03
Authors
Rapie, Jalilah
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Publisher
Universiti Sains Malaysia
Abstract
Dendrobium crumenatum is one of the families of Orchidaceae, produces white, fragrant flower with a yellow tinted throat that commonly called as pegion orchid. This orchid is the most common in Malaysia found naturally on trees in the tropical forests. Most of the orchids were depend on orchid mycorrhizal fungi to establish and survive. Thus, the main objective of this study is to understand the relationship of the mycorrhizal fungi in epiphytic orchid roots of Dendrobium crumenatum and bark of Rain Tree (Samanea saman). The relationship of the mycorrhizal fungi in epiphytic orchid roots of (D. crumenatum) and bark of Rain Tree (S. saman) can be determined by observing the presence of peloton in the root of epiphytic orchid. The presence of peloton in the roots was observed by using two method of sectioning; hand section and microtome sectioning. A total 251 isolates of fungi were successfully isolated from roots of epiphytic orchid and bark of Rain Tree with and without growing orchid. Only 17 isolates was identified as Rhizoctonia-like fungi, in which fourteen isolates from root of epiphytic orchid and three isolates from bark of Rain Tree with growing orchid. Rhizoctonia-like was isolated neither from root of epiphytic orchid, nor bark of Rain Tree without growing orchid. The 17 isolates of Rhizoctonia-like were grouped into four groups that is Group I, II, III, and IV based on their morphological characteristic and anastomosis analysis. Number of nuclei present in immature cells showed that all the four groups of Rhizoctonia-like fungi were binucleate. Besides that, anatomosis analysis of Rhizoctonia-like fungi also showed that their successful hyphal fusion corresponded with the group determined by morphological characteristic.
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Keywords
Orchid