Phytochemical screening and natural killer cells immunomodulation effects of pereskia bleo leaves extract on cervical cancer cells
| dc.contributor.author | Salleh, Siti Farhanah Mohd | |
| dc.date.accessioned | 2020-12-21T08:30:18Z | |
| dc.date.available | 2020-12-21T08:30:18Z | |
| dc.date.issued | 2020-08 | |
| dc.description.abstract | Pereskia bleo is a leafy and edible plant, locally known as 'Pokok Jarum Tujuh Bilah" which has anti-cancer properties. This study purposed to elucidate the underlying mechanism of this plant as anti-cancer in inducing cell death as well as to evaluate its immunostimulatory effects on Natural Killer cells (NK cells) as a potential additional anti-cancer effect. In this study, the leaves of P. bleo were extracted using propenyl) and hexadecanoic acid. PBEA exhibited the lowest IC50 value (14.37 ± 8.40 μg/ml) indicated the strongest cytotoxic effect selectively on cervical cancer cells (HeLa). The cell cycle analysis showed inhibition of cell proliferation at G0/G1 phase in PBEA treated HeLa cells as evidenced by a significant accumulation of the cells at this phase (P<0.05). Morphological examination on PBEA treated HeLa cell showed the presence of fragmented nuclei and condensation of chromatin while apoptosis was detected in the Annexin V/PI assay. Analysis of apoptotic proteins revealed a significant upregulation of pro-apoptotic proteins (Bax, p53 and caspase-3) while downregulation of anti-apoptotic protein Bcl-2 (P<0.05) in PBEA treated HeLa cells. Meanwhile, NK cells proliferation at 24 h was found significantly increased compared to 48 h and 72 h of PBEA treatment (P<0.05). Apoptosis of HeLa cells was markedly increased in PBEA treated NK cells from cancer patients. This extract also enhanced granzyme B and IFN- epression in NK cells from cancer paiens. Thus our findings demonstrated that PBEA induced cell death in the cervical cancer cells (HeLa) and stimulate activation of NK cells from cervical cancer patients which enhanced cytotoxic effect against HeLa cells. These results provide some insight into the effectiveness of P. bleo as a potential chemopreventive agent which open up for further studies. different techniques and solvent polarities, and subsequently subjected to GC-MS analysis. The extracts were tested for its cytotoxic effects on HeLa, MDA-MB-231, SW480 and NIH/3T3 cell lines using MTT assay. The most cytotoxic extract and its corresponding cancer cell lines were investigated for their cell death induction through cell cycle arrest, Annexin V/PI assay and measurement of apoptotic proteins using flow cytometry. NK cells were exposed to different concentrations of ethyl acetate extract of P. bleo leaves (PBEA) and its proliferation rate was determined via MTT assay. NK cells from healthy individuals and cervical cancer patients were treated with 14.4 μg/ml of PBEA and co-cultured with target cells for 24 h to evaluate its cytotoxic activity. Target cells death was identified by flow cytometry while ELISA assay was performed to determine the production of perforin, granzyme B, IFN- and IL-2. Results showed the presence of terpenoids, sterols, alkaloids, flavonoids, phenols, fatty acids and vitamin E in the extracts of P. bleo leaves together with new compounds namely (-)-Loliolide, neophytadiene, -ocopherol, -tocopherol, squalene, 4H-Pyran- 4-one,2,3-dihydro-3,5-dihydroxy-6-methyl, 4-vinyl-syringol, phenol,2-methoxy-4-(1- | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/10785 | |
| dc.language.iso | en | en_US |
| dc.subject | anti-cancer | en_US |
| dc.title | Phytochemical screening and natural killer cells immunomodulation effects of pereskia bleo leaves extract on cervical cancer cells | en_US |
| dc.type | Thesis | en_US |
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