LC-MS/MS Analysis Of Plasma Homocysteine And Investigation On The Association Of Homocysteine Levels With Diabetic Peripheral Neuropathy

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dc.contributor.authorLim, Luen Hui
dc.date.accessioned2017-01-19T01:40:33Z
dc.date.available2017-01-19T01:40:33Z
dc.date.issued2015-10
dc.description.abstractA simple LC-MS/MS method was developed to measure homocysteine level in human plasma collected from diabetic patients. This method which involves simple sample preparation showed good precision, specificity, sensitivity and accuracy. Thus, it can be used to screen a vast number of clinical samples. This method‘s results were compared with commercial laboratory results derived from the immunoassay method. Both methods showed good accuracy with low mean difference. Of the 260 patients recruited with peripheral neuropathy, 26% showed hyperhomocysteinemia (homocysteine ≥15μM). The male gender was more prominent in the hyperhomocysteine group. Homocysteine level was directly associated with creatinine, while homocysteine level was inversely correlated with the vitamin status (B12 and folic acid). No correlation was observed between homocysteine level and severity of neuropathy in the present study. However, age, gender and duration of diabetes were associated with neuropathy severity. Higher age group patient and the male gender recorded higher means in neuropathy impairment score. A higher mean neuropathy impairment score was also observed with longer duration of diabetes.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/3498
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectHomocysteineen_US
dc.titleLC-MS/MS Analysis Of Plasma Homocysteine And Investigation On The Association Of Homocysteine Levels With Diabetic Peripheral Neuropathyen_US
dc.typeThesisen_US
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