Protein Enrichment Of Pycnoporus Sanguineus For Silver Nanoparticles Synthesis
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Date
2014-02
Authors
Chan, Yen San
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Controlling sizes of nanomaterials are attracting a great deal of attention because of
their unit properties for achieving specific processes especially in biological and
medical applications. In this context, Ag which possesses good antimicrobial effects
is of interest. A simple and effective approach for the synthesis of Ag nanoparticles
by reduction mechanism of proteins synthesized by several species of white rot fungi
was studied. Pycnoporus sanguineus was found to produce smallest Ag nanoparticles
with degree of monodispersity. A Box-Behnken design was employed to optimize a
fermentation medium for the production of protein by the selected fungi. The four
variables involved in the study were glucose, malt extract, yeast extract and pH. The
optimized medium contained 8.39 gL-1, 18.34 gL-1, 14.71 gL-1 of yeast extract,
glucose and malt extract respectively with initial pH 6.66; and produced the
maximum protein at 0.858 gL-1. Kinetic growth of the fungi, glucose consumption
and product formation of the tested fungus were also reported. It is showed that at
initial glucose concentration of 20gL-1, Richard-modified logistic model (R2
=0.9839) for microbial growth, modified Gompertz model (R2 =0.9610) for product
formation and first order non-growth associate (R2 =0.9736) for glucose
consumption were fittd well with the experimental data. Sequential optimization
strategy based on one-factor-at-a-time (OFAT) method and statistical experimental
design were employed to optimize the process parameters for the enhancement of Ag
nanoparticles synthesis. Based on the OFAT method, three significant factors
influencing the size of Ag nanoparticles such as AgNO3 concentration, incubation
temperature and agitation speed.
Description
Keywords
Protein Enrichment , Pycnoporus Sanguineus