Drug metabolism and toxicity studies of orthosiphon stamineus, benth (misai kucing) in rats

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dc.contributor.authorJin Han, Chin
dc.date.accessioned2015-07-30T03:46:26Z
dc.date.available2015-07-30T03:46:26Z
dc.date.issued2006
dc.description.abstractOrthosiphon stamineus, Benth (Family: Lamiaceae) or locally known as Misai Kucing, is widely used in Malaysia for treating hypertension, urinary system ailments and kidney stone disease. The objective of this study is to investigate the effect of standardised methanol extract of Misai Kucing on phase I and phase II hepatic drug metabolising enzymes in Sprague-Oawley (SO) rat. Aminopyrine, p-nitrophenol (pNP) and 1-chloro-2,4-dinitro benzene (CONB) was used as a substrate to determine the aminopyrine N-demethylase, UOP-glucuronosyltransferase (UGT) and glutathione-Stransferase (GST) activity respectively in SD rat liver. Isolated hepatocytes, liver cytosolic fraction and liver microsomes from SO rats were prepared using the collagenase perfusion technique and differential centrifugation technique. The possible toxic and anti-hepatotoxicity effects of standardised of methanol extract of Misai Kucing on SO rats were also examined. Results obtained showed that most of the UGT and GST activity in normal and diabetic SO rat liver were significantly affected by standardised methanol extract of Misai Kucing. Only a few groups of normal and diabetic SO rats showed significant effect on aminopyrine metabolism in hepatocytes in the presence of standardised methanol extract of Misai Kucing. The possible mechanism of action ofthe in vitro effect of 1 mg/ml of standardised methanol extract of Misai Kucing on aminopyrine metabolism in normal old male SD rat hepatocytes was probably mediated through the activation of G-protein and protein kinase C while the in vitro effect of 0.001 mg/ml of Misai Kucing extract on normal young female SO rat hepatocytes was probably mediated through the activation of protein kinase A. The LOse of standardised of methanol extract of Misai Kucing could not be determined in this study. No lethality incident and any toxic effects were observed in normal young male and female SO rats after being treated with 0.5, 1.0, 3.0 and 5 g/kg of standardised methanol extract of Misai Kucing. Pre-treatment with standardised methanol extract of Misai Kucing increased the percentage of hepatocytes viability and decreased some of the serum AST, ALT, ALP and GGT level in 2g/kg of acetaminophen induced liver damage in adult male and female SO rats. In conclusion, standardised methanol extract of Misai Kucing had a greater influence on UGT and GST activity than the aminopyrine N-demethylase activity in SO rat liver. Standardised methanol extract of Misai Kucing at 0.5 g/kg is classified as not-observable effect level (NOEL) and it exhibited anti-hepatotoxicity effect on acetaminophen-induced liver injury in adult male and female SO rats.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/971
dc.language.isoenen_US
dc.subjectOrthosiphon stamineusen_US
dc.subjectMisai Kucingen_US
dc.titleDrug metabolism and toxicity studies of orthosiphon stamineus, benth (misai kucing) in ratsen_US
dc.typeThesisen_US
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