Synthesis of curcumin encapsulated alginate microspheres for drug delivery system
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Date
2019-10-01
Authors
Nguyen Thi Thanh Uyen
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Abstract
Curcumin loaded alginate microspheres (Cur-AMs) have been potentially used in drug delivery system for therapeutic treatment. However, the ideal Cur-AMs system requires the microspheres with a mean diameter less than 200 μm remains a challenge. Therefore, in this research, alginate microspheres (AMs) and Cur-AMs were synthesized via the emulsification/gelation method to tailor its particle sizes. The synthesized parameters include alginate concentration, drug content, water in oil ratio, cross-linking time and stirring speed were investigated. Scanning electron microscope (SEM) observation showed that the AMs and Cur-AMs obtained were in spherical shapes with mean particle size ranging from 20.3 μm to 77.4 μm which is within the targeted value. The optimum formulation for AMs formation was 4% alginate concentration, water in oil ratio of 1/10, crosslinking time for 90 min and stirring speed at 300 rpm. The optimum formulation of Cur-AMs was similar to AMs with alginate/curcumin ratio at 1/100. Encapsulation efficiency (EE) of curcumin obtained was 50.68%. Drug release profile showed slow release of curcumin over 28 days. In vitro antimicrobial activity test using S. aureus ATCC 12600 and E. coli ATCC 25922 microorganism showed that curcumin are more susceptible to S. aureus ATCC 12600 and resistance to E. coli ATCC 25922. Differential Scanning calorimetry (DSC), X-ray diffraction (XRD) and Fourier transform infrared (FTIR) analysis showed that there was interaction between curcumin and alginate polymer which leads to the amorphous form of curcumin in Cur-AMs. In summary, the AMs and Cur-AMs were successfully synthesized via emulsification/gelation method. The obtained Cur-AMs showed potential for application in drug delivery therapeutic treatment system.