Pembangunan kultur ampaian sel misai kucing (orthosiphon stamineu benth) untuk penghasilan asid rosmarinik
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Date
2009
Authors
Fung Liang, Lim
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Abstract
Elite cell lines of misai kucing ( Orthosiphon stamineus Benth) were selected
from in vitro plantlets derived from different locations based on plantlets height that
can produce the optimum rosmarinic acid. Three cell lines derived from mother plants
from Air !tam and Butterworth and two cell lines derived from Relau were selected
based on their height. Cell suspension cultures derived from fast growing in vitro
plantlets from Air Itam produced the highest content of rosmarinic acid (5.0%) after 12
days of culture. The selected cell lines grew well in 100 ml to 1000 ml Erlenmeyer
flasks contained culture medium 30% of the flasks volume. Elicitors were applied to
the selected cell lines and the in vitro plantlets of misai kucing to enhance the
production of rosmarinic acid. Only 45 g/1 of sucrose added into the liquid cell
suspension culture medium (MS + 1.0 mg/1 2,4-D and 1.0 mg/1 NAA) significantly
enhanced the highest production of the rosmarinic acid (5.5%) compared to the control
(4.9%). The free radical scavenging activity and total phenolic compound in the cells
increased after the cells had been treated with various type of elicitors. Treatment with
1.50 g/1 yeast extract, 150 mg/1 chitosan, 2.0 g/1 sodium chloride and 45 g/1 sucrose
successfully increased the free radical scavenging activity of the misai kucing cells.
Meanwhile, the addition of 1.50 g/1 yeast extract, 100 mg/1 and 150 mg/1 chitosan and
45 g/1 sucrose into the culture medium increased the total phenolic compound in the
misai kucing cells. Application of 4.0 g/1 yeast extract, 60 mg/1, 120 mg/1 and 240 mg/1
chitosan and 1.0 g/1 and 4.0 g/1 casein hydrolisate successfully increased the production
of rosmarinic acid in the in vitro plantlets of misai kucing.
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Keywords
Kultur ampaian sel misai kucing , (Orthosiphon stamineu benth) , penghasilan asid rosmarinik