SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL PROPERTIES OF NICKEL AND IRONCARBOXYLATE COMPLEXES
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Date
2010-08
Authors
CH'NG, CYNN DEE
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Abstract
In this research, nine Ni(II) complexes were successfully synthesized using nickel(II)
chloride hexahydrate, NiClz.6H20 with amino acids (Glycine, l3-alanine and Lhistidine),
carboxylic acids (2-picolinic acid, 3,5-dinitrobenzoic acid, 4-
aminobenzoic acid and pyrazine-2-carboxylic acid) and with dicarboxylic acids
(maleic acid and pyridine-2,6-dicarboxylic acid). Besides, two Fe(I1) complexes and
one Fe(III) complexes were also successfully synthesized. Fe(II) complexes were
synthesized using iron(II) chloride 4-hydrate, FeClzAH20 with pyrazine-2-
carboxylic acid and maleic acid respectively. While Fe(III) complex was synthesized
using ferric chloride anhydrous, FeCh with pyridine-2,6-dicarboxylic acid.
Stoichiometry ratio of metal to ligand being used is 1 :2. These complexes were
characterized by determination of melting point, solubility, conductivity
measurement, Fourier Transform Infrared Spectroscopy (FTIR), CHN microanalysis,
Analysis of Atomic Absorption Spectroscopy (AAS), Ultraviolet-Visible
Spectrometry (UV-Vis), Cyclic Voltammetry (CV), Thermogravimetric Analysis
(TGA) and X-ray crystallography. Besides that, the interaction between the metal
complexes and pBR322 DNA will be investigated by gel electrophoresis experiments,
while the cytotoxic activity of the complexes was tested by MTT Assay. FT-IR
analysis result shows that the carboxylate group in all of the ligand were coordinated
to the central metal ion monodentately. The UV -Vis analysis shows that the
oxidation state for nickel complexes is +2 and its configuration system is d8
meanwhile the oxidation state for Fe(II) and Fe(III) complexes are +2 and +3 and
configuration systems are d6 and d5 respectively. The CV analysis shows that all the
complexes have reversible redox properties which are very important in biological
uses. Gel electrophoresis experiments show that all the complexes successfully
promote the cleavage of plasmid DNA, producing single and double DNA strand
breaks. In MTT assay results, all of the complexes showed cytotoxic activity against
HepG2 cancer cell lines.
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Keywords
SYNTHESIS , BIOLOGICAL