SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL PROPERTIES OF NICKEL AND IRONCARBOXYLATE COMPLEXES
| dc.contributor.author | CH'NG, CYNN DEE | |
| dc.date.accessioned | 2016-01-22T08:03:40Z | |
| dc.date.available | 2016-01-22T08:03:40Z | |
| dc.date.issued | 2010-08 | |
| dc.description.abstract | In this research, nine Ni(II) complexes were successfully synthesized using nickel(II) chloride hexahydrate, NiClz.6H20 with amino acids (Glycine, l3-alanine and Lhistidine), carboxylic acids (2-picolinic acid, 3,5-dinitrobenzoic acid, 4- aminobenzoic acid and pyrazine-2-carboxylic acid) and with dicarboxylic acids (maleic acid and pyridine-2,6-dicarboxylic acid). Besides, two Fe(I1) complexes and one Fe(III) complexes were also successfully synthesized. Fe(II) complexes were synthesized using iron(II) chloride 4-hydrate, FeClzAH20 with pyrazine-2- carboxylic acid and maleic acid respectively. While Fe(III) complex was synthesized using ferric chloride anhydrous, FeCh with pyridine-2,6-dicarboxylic acid. Stoichiometry ratio of metal to ligand being used is 1 :2. These complexes were characterized by determination of melting point, solubility, conductivity measurement, Fourier Transform Infrared Spectroscopy (FTIR), CHN microanalysis, Analysis of Atomic Absorption Spectroscopy (AAS), Ultraviolet-Visible Spectrometry (UV-Vis), Cyclic Voltammetry (CV), Thermogravimetric Analysis (TGA) and X-ray crystallography. Besides that, the interaction between the metal complexes and pBR322 DNA will be investigated by gel electrophoresis experiments, while the cytotoxic activity of the complexes was tested by MTT Assay. FT-IR analysis result shows that the carboxylate group in all of the ligand were coordinated to the central metal ion monodentately. The UV -Vis analysis shows that the oxidation state for nickel complexes is +2 and its configuration system is d8 meanwhile the oxidation state for Fe(II) and Fe(III) complexes are +2 and +3 and configuration systems are d6 and d5 respectively. The CV analysis shows that all the complexes have reversible redox properties which are very important in biological uses. Gel electrophoresis experiments show that all the complexes successfully promote the cleavage of plasmid DNA, producing single and double DNA strand breaks. In MTT assay results, all of the complexes showed cytotoxic activity against HepG2 cancer cell lines. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/1691 | |
| dc.subject | SYNTHESIS | en_US |
| dc.subject | BIOLOGICAL | en_US |
| dc.title | SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL PROPERTIES OF NICKEL AND IRONCARBOXYLATE COMPLEXES | en_US |
| dc.type | Thesis | en_US |
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