Characterization Of The Anti-Angiogenesis Activity Of Labisia Pumila And Identification Of Its Molecular Constituents That Contribute To Its Biological Activity

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dc.contributor.authorAbdul Samad, Nozlena
dc.date.accessioned2019-10-07T08:14:24Z
dc.date.available2019-10-07T08:14:24Z
dc.date.issued2010-07
dc.description.abstractAngiogenesis is a process of new blood vessel formation. Inhibition of angiogenesis is considered one of the most promising strategies in treating variety of illnesses including cancer. Labisia Pumila var. alata (Myrsiaceae) or locally known as ‘kacip fatimah’, a lowland plant, is used widely by the Malays in Peninsular Malaysia to treat many health problems and ailments. A literature search did not reveal any reports of its anti-angiogenesis activity. To date this is the first study on anti-angiogenesis activity of Labisia pumila. The aim of this study was to investigate anti-angiogenesis activity, anti-oxidant properties, total phenolic content and chemical constituents of Labisia pumila leaves extracts and fractions. The plant was pulverized and extracted successively with petroleum ether (PE), chloroform (CE), methanol (ME) and water (WE). The anti-angiogenesis activity of different extracts (PE, CE, ME and WE) of Labisia pumila leaves was studied using rat aortic ring assay. The study showed that Labisia pumila leaves extracts significantly inhibited microvessels outgrowth of extracts. The methanolic extract gave the most significant anti-angiogenesis activity (P<0.05). This extract was further fractionated into three fractions, ethyl acetate fraction (EAF), n-hexane fraction (NF) and water fraction (WF). WF had the highest anti-angiogenesis level, which was lower than the level of the methanol extract (ME). These two samples were found to be non-cytotoxic in the MTT assay against human umbilical vein endothelial cells (HUVEC). Both samples showed no significant cytotoxic activity towards selected cancer cell lines. ME and WF were also found to inhibit the endothelial tube formation in the HUVEC tube formation assay. Labisia pumila methanol extract and water fraction demonstrated the inhibition of VEGF protein expression level.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8962
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectAnti-Angiogenesis Activityen_US
dc.subjectLabisia Pumilaen_US
dc.titleCharacterization Of The Anti-Angiogenesis Activity Of Labisia Pumila And Identification Of Its Molecular Constituents That Contribute To Its Biological Activityen_US
dc.typeThesisen_US
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