In Vitro Antibacterial And Wound Healing Properties Of A Standardized Polyphenols-Rich Fraction Of Dicranopteris Linearis (BURM.) Underwood
| dc.contributor.author | Ponnusamy, Yasodha | |
| dc.date.accessioned | 2017-01-16T01:21:17Z | |
| dc.date.available | 2017-01-16T01:21:17Z | |
| dc.date.issued | 2016-03 | |
| dc.description.abstract | Dicranopteris linearis (Burm.)Underwood is a ground creeping fern used traditionally to treat various skin related ailments, such as boils, ulcers and external wounds. To date, no scientific studies have been carried out to evaluate its ethnopharmacological use in wound infection in a systematic manner. In the present study, the MeOH extract of D. linearis was fractionated with the bioactive constituents following a bioactivity-guided approach. A fraction (F5) of the extract was obtained and it was found to possess significant antioxidant and antibacterial activity against S. aureus and P. aeruginosa. Analysis was further carried out to identify the chemical constituents of this active fraction. Isolation of the major constituents was carried out using a combination of reversed-phase and normal-phase silica gel chromatographic techniques. Two phenolic compounds which are taxonomically significant to D. linearis, namely dichotomain A (2) and dichotomain B (3), and two new compounds, 4-vinyl phenol-1-O-(2‟-O-α-L-rhamnopyranosyl)-β- D-glucopyranoside (5) and 4-vinyl phenol-1-O-(4‟-O-α-L-rhamnopyranosyl)-β-Dglucopyranoside (6) together with two other known compounds kaempferol 3-O-β- D-glucopyranoside (1) and kaempferol 3-O-β-D-glucosyl (1→2)-β-glucoside (4) were obtained. Characterization of the constituents was carried out using various spectroscopic techniques such as UV, IR, NMR and MS. In order to determine the content of F5 and to standardize the fraction, a novel HPLC–DAD method was developed and validated using the isolated compounds as analytical markers. This method was found to be accurate and precise, with an LOQ of 1.56 μg/mL for compounds 1, 4, 5& 6 and 15.6 μg/mL for compound 2. The contents of the marker compounds were found to be 3.1% for compound 1, 3.4% for compound 2, 3.4 % for compound 4, and 26.8 % for compound 5 & 6. Antibacterial and wound healing potential of the standardized F5 fraction of D. linearis was subsequently investigated using several in vitro assays. From the time killing assay, minimum inhibitory concentration (MIC) assay and scanning electron microscopic images, F5 was found to exert bacteriostatic effect against S. aureus and P. aeruginosa. Moreover, F5 was also able to potentiate the antibacterial effect of few conventional antibiotics, namely, chloramphenicol, penicillin G and ampicillin against S. aureus and MRSA. On the other hand, F5 was able to enhance the proliferation of fibroblast cells and induce cell migration in the scratch-wound assay. In addition, it protected the cells against hydrogen peroxide induced oxidative stress and was non-cytotoxic towards both human and mouse fibroblast cell lines up to the tested concentrations of 500μg/mL. Taken together, the present study provided novel information to support the traditional use of this plant for skin infections. In vivo studies are warranted to further explore the potential of D. linearis as a wound healing agent. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/3438 | |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Sains Malaysia | en_US |
| dc.subject | Antioxidant and antibacterial | en_US |
| dc.title | In Vitro Antibacterial And Wound Healing Properties Of A Standardized Polyphenols-Rich Fraction Of Dicranopteris Linearis (BURM.) Underwood | en_US |
| dc.type | Thesis | en_US |
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