Screening And Characterisation Of Potential Lipase Producing Strains From Arctic Samples

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dc.contributor.authorRamle, Zakiah
dc.date.accessioned2019-01-24T08:35:17Z
dc.date.available2019-01-24T08:35:17Z
dc.date.issued2018-01
dc.description.abstractPsychrotolerant microorganisms are widespread in natural cold environments ranging from deep sea to polar region and have a synergistic mechanism that allows them to adapt living in the cold. Possessing evolved cold active enzymes is one of the basic strategies of adaptation. Among the various cold enzymes is cold active microbial lipase, an enzyme that is able to hydrolyse triglycerols releasing fatty acids. Microbial lipases have gained notable attention for their widespread uses in industries. However, bulk production of enzymes direct from original microbe source is not feasible due to high maintenance cost thus will not be able to cater to high industrial demands. Developments for efficient lipase productions are still necessary for lipase biocatalysis to rise to its full potential. This study was conducted to screen and identify suitable psychrotolerant lipase producing strain to be expressed in a heterologous host expression system before characterising the recombinant protein. With that in mind, six microorganisms labelled as ARB 1A, ARB 1B, ARB 1C, ARB 10, ARC 8A and ARC 8B’s DNAs were extracted via modified CTAB method and applied to 16S rDNA sequence analysis. Gram staining revealed five out of six isolates were Gram negative. They were identified as bacteria belonging to four genera Pseudomonas sp., Arthrobacter sp., Acinetobacter sp., and Janthinobacterium sp., and were screened for lipase activity. Lipase activity was detected using sensitive and specific plate assay using olive oil as substrate. ARB 1B, ARB 10 and ARC 8A were tested as lipase positive. ARB 10, having 97% similarity to Pseudomonas sp. was selected for cloning. Lipase gene was amplified by PCR method using specific set of primers that was designed to target the gene of interest.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/7656
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectScreening and characterisation of potential lipaseen_US
dc.subjectproducing strains from arctic samplesen_US
dc.titleScreening And Characterisation Of Potential Lipase Producing Strains From Arctic Samplesen_US
dc.typeThesisen_US
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