Isolation Of Chavibetol And Hydroxychavicol And Selected Anti-Obesity Studies Of Standardized Piper Betle Linn. Leaf Extracts

dc.contributor.authorMohamad, Nurul Najwa
dc.date.accessioned2017-01-18T02:55:24Z
dc.date.available2017-01-18T02:55:24Z
dc.date.issued2016-09
dc.description.abstractPiper betle L. has been extensively used in traditional herbal remedies in Asia. Nevertheless, this plant still lacks of data especially on their quality, safety and efficacy. This study has been conducted to provide information on the standardization of Piper betle leaves. The study was divided into three parts: quality and safety of raw materials of Piper betle leaves, standardization of Piper betle extracts using isolation compounds and anti-obesity studies. Dry powdered leaves of Piper betle were determined to evaluate the content of heavy metals and the microbial limit test (MLT). This plant was found to be in the range of the acceptable limit of four main heavy metals Cd (0.3ppm), Hg (0.5 ppm), Pb (10 ppm) and As (5.0 ppm) and free from microbial contamination. The preliminary phytochemical screening using TLC on the ethanol, 50% ethanol and water extracts showed the presence of terpenoids, flavonoids, phenols, sterols, coumarins and saponins. Two compounds namely chavibetol (CHV) and hydroxychavicol (HC) were isolated and identified by UV, FTIR, HPTLC, HPLC, GC-MS and NMR data. The chemical profile using spectroscopic and chromatographic techniques such as UV, FTIR, HPTLC and GC-MS were used in the standardization. Chavibetol and hydroxychavicol were carried out as markers to examine the qualitative and quantitative analysis. An isocratic HPLC system has been developed and validated for the quantification of chavibetol and hydroxychavicol of Piper betle extracts. The HPLC analysis of three different extracts of Piper betle exhibited varying concentration of chavibetol (0.0219 - 0.1020 μg/mL) and hydroxychavicol (0.1371 - 0.3424 μg/mL). The extracts also were determined in the evaluation of total flavonoids, total phenolics, total glycosaponins, total polysaccharides and total proteins. An ethanol extract of Piper betle was found to be the highest content of flavonoids (125.21 μg/mL), phenolics (361.26 μg/mL) and proteins (7.45%), while the 50% ethanol extract have demonstrated the highest amount of glycosaponins (35.36%) and polysaccharide (46.44%). Ethanol, 50% ethanol and water extracts of Piper betle leaf were screened for their anti-lipase activity using porcine pancreatic lipase (PPL) and 2,4-dinitrophenyl butyrate (p-NPB) as an enzyme and artificial substrate. The 50% ethanol extract of Piper betle indicated anti-lipase activity. The anti-obesity and anti-hyperlipidemic activities of 50% ethanol extract of Piper betle were examined using high fat diet (HFD) induced hyperlipidemic rats as a model. The data demonstrated 50% ethanol extract of Piper betle significantly (P<0.05 and P<0.01, respectively) lowered the percentage of total body weight increased and body mass index (BMI). The findings also exhibited this extract was significantly (P<0.01) decreased the level of total cholesterol (TC) and low density lipoprotein (LDL) while indicating an increased level of high density lipoprotein (HDL). Results of the study indicated that the developed HPLC methods can be applied for standardizations of Piper betle extracts. While, 50% ethanol extract has shown positive result as an anti-lipase, anti-obesity and anti-hyperlipidemic agent.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/3482
dc.subjectIsolation Of Chavibetol And Hydroxychavicol And Selected Anti-Obesityen_US
dc.subjectStudies Of Standardized Piper Betle Linn.en_US
dc.titleIsolation Of Chavibetol And Hydroxychavicol And Selected Anti-Obesity Studies Of Standardized Piper Betle Linn. Leaf Extractsen_US
dc.typeThesisen_US
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