Assessment Of Proliferation Rate In Breast Cancer By Ki-67 Labeling Index
Loading...
Date
2017-06
Authors
Hussin Khan, Ibrahim
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Introduction: Breast cancer is the commonest cancer in women. One of the characteristics
of breast cancer is its association with the limitless replicative potential of the malignant
cells. This cellular proliferation can be gauged by looking at Ki-67 labelling index, which is
a type of proliferation biomarker. This study aims to compare the Ki-67 labelling index
between the manual and the automated counting methods with various cut-off values.
Methods: Eighty-eight breast cancers that met the inclusion criteria from year 2014 to
2016 were recruited in this study. Clinicopathological data were extracted from the
pathology reports. Immunohistochemistry for Ki-67 was performed on tissue sections.
Manual counting and automated counting using ImmunoRatio software were performed to
assess the Ki-67 labelling index. Two different cut-off points, i.e. <14% and <20% were
addressed.
Results: Most of the patients diagnosed were more than 40 years old and majority
were Malays. Ki-67 labelling index by manual counting correlated with automated method
(r=0.99; p<0.001). When shifting the cut-off value from <14% to <20%, as compared to
manual count, automated method resulted in a larger number of Luminal B breast cancers
re-classified into Luminal A breast cancers. Using <20% as cut-off point, 3 cases that were
assessed as >20% by manual counting was downgraded as <20% by automated method.
Conclusion: The automated method is reliable in assessing proliferation rate as compared
to the manual method, although a minority of cases showed discrepancy between these two
methods
Description
Keywords
Assessment of proliferation rate , in breast cancer By ki-67 labeling index