Cloning and characterization of bovine growth hormone (BGH) gene from local gaur(Seladang)
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Date
2005-03
Authors
Jusoh, Abdul Rashid
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
Abstract
Bovine growth honnone gene (bGH) is well know among the scientist all over the
world. Various types of bGH gene has been publish from various bovine species.
Expression of that sequence has produced a valuable protein that is known as bovine
growth hormone. These hormone play important role in regulated the differentiation,
growth and metabolism of cattle. Has been proven that, this hormone play important role in
increasing the production of milk. In searching full information about that gene, many bGH
sequence has been explore in different bovine species
However the sequence of bGH gene from Bos gaurus hubbacki (local Malaysian
gaur) still not explore yet. To grab these opportunity my supervisor with collaboration of
Seladang Conservation Centre has proposed these study. In this study we try to clone and
characterize bGH gene from genomic DNA. The genomic DNA was extracted from gaur
tissue using phenol chloroform method proposed by Maniatis et al. (1989). The bGH gene
has been amplified using polymerase chain reaction. Amplified product then has been
further clone into T A Cloning vector. The recombinant plasmid has been transfonned into
E.coli DH5a. The cloned DNA finally has been submitted for sequencing and sequence
analysis of the gene has been carried out.
This study reveals that, bGH gene has been successful cloned from genomic DNA
of local gaur. The size of the cloned gene is 1208 bp and has high similarity with bGH gene
from other bovine species reported in GenBank.
Description
Keywords
biomedicine