Physicochemical Properties And Prebiotic Potential Of Native, Resistant And Hcl-Resistant Starches From Sago (Metroxylon Sagu)
| dc.contributor.author | Tan, Zi Ni | |
| dc.date.accessioned | 2017-02-01T00:51:20Z | |
| dc.date.available | 2017-02-01T00:51:20Z | |
| dc.date.issued | 2016-04 | |
| dc.description.abstract | Resistant starch type III (RS3) was produced from sago (Metroxylon sagu) and evaluated for its physicochemical properties and potential as a prebiotic. A sample with 35.7% RS3 content (designated as sago RS) was produced when the native sago starch was suspended in distilled water, gelatinized by autoclaving at 121 °C for 1 h, followed by debranching with 20 U pullulanase per g starch at 60 °C for 24 h, autoclaved again at 121 °C for 1 h before storage at 4 °C for 24 h. RS3 content was further increased with the treatment of sago RS with 0.5 M HCl at 60 °C (sample designated HCl-sago RS) to 63.8%. Granules of sago RS and HCl-sago RS had B-type X-ray diffraction pattern, high peak temperatures (143.7 °C and 146.5 ºC, respectively) and showed irregular and rough surface structure. While granules of native sago starch had C-type diffraction pattern, peak temperature of 74.6 °C and smooth granular surface. The solubility and the swelling power of HCl-sago RS samples were 14.9% and 1.94 g/g, respectively, which were lower than that of sago RS (27.4% and 2.82 g/g, respectively). Sago RS and HCl-sago RS samples were resistant to 180 min hydrolysis by gastric acidity at pH 1 to 4 with less than 0.85% hydrolyzed. Both samples were also resistant toward hydrolysis by gastrointestinal tract enzymes and intestinal absorption with 96.8% and 98.7% of RS3 were recovered respectively after 3.5 h digestion and overnight dialysis at 37 °C. Sago RS and HClsago RS acted selectively, by increasing the growth of rat intestinal bacteria (lactobacilli and bifidobacteria) while decreasing the growth of detrimental bacteroides, clostridia and enterobacteria. The prebiotic indexes of sago RS, HClsago RS, oligofructose and inulin were +12.19, +4.75, +9.45 and +6.82, respectively. Butyric acid production by rat faecal culture was higher in media with Sago RS and HCl-sago RS than with oligofructose and inulin. The activity of β-glucuronidase were reduced by sago RS and HCl-sago RS. Contrary, native sago starch supported the growth of both beneficial and detrimental bacteria. Sago RS and HCl-sago RS were the better growth substrate for Lactobacillus plantarum FTCC0350 as compared with FOS and inulin. Lactic and acetic acid production by Lactobacillus plantarum FTCC0350 was higher in media with sago RS and HCl-sago RS. In conclusion, sago RS and HCl-sago RS exhibited prebiotic characteristic and they are potential prebiotic | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/3627 | |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Sains Malaysia | en_US |
| dc.subject | Sago (Metroxylon sagu) | en_US |
| dc.title | Physicochemical Properties And Prebiotic Potential Of Native, Resistant And Hcl-Resistant Starches From Sago (Metroxylon Sagu) | en_US |
| dc.type | Thesis | en_US |
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