Molecular Characterization Of Fusarium Oxysporum F. Sp. Cubense (Foc) In Malaysia
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Date
2009-05
Authors
Leong, Sau Kueen
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
A total of 59 Fusarium oxysporum f. sp. cubense (FOC) isolates were analysed
using ERIC-PCR, RAMS and RFLP-IGS. The three molecular techniques showed
intraspecific variation among the FOC isolates, whereby 34 ERIC banding patterns,
33 IGS haplotypes and 13 – 26 microsatellite banding patterns were generated. The
banding patterns generated using each technique were highly variable, but showed
similarities among the FOC isolates. UPGMA cluster analyses based on ERIC-PCR
(15.4% - 100%) and RAMS (11.1% - 100%) showed similarity values very close to
those generated based on combined data (11.0% - 100%) of the three techniques,
while RFLP-IGS showed higher similarity values of approximately 60% - 100%
among the FOC isolates.
Description
Keywords
Molecular Characterization , Fusarium Oxysporum F. Sp. Cubense (Foc) In Malaysia