The effects of tualang honey on nociceptive responses in the thalamus of prenatally stressed male rats offspring
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Date
2018-04
Authors
Hasim, Hidani
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
Abstract
Various mechanisms have been postulated to contribute to alteration of
nociceptive responses in offspring exposed to prenatal stress; however not a single
study has investigated the possible role of thalamus in the nociceptive modulation.
The first aim of this study was to investigate the effects of prenatal stress on
nociceptive behaviour, morphological changes, level of oxidative stress parameters
and NMDA receptors in the thalamus of prenatally stressed male rats offspring. The
second aim was to determine the effects of Tualang honey on these parameters.
Thirty-three Sprague Dawley pregnant rats were randomised into control, stress and
stress-treated with Tualang honey groups. Oral administration of Tualang honey
(1.2g/kg body weight/day) or distilled water was given to the corresponding pregnant
dams. Restraint stress was applied to the stress and stress-treated groups from day 11
of pregnancy until delivery. Maternal faeces were collected (on day 11 and 21) to
measure the level of corticosterone. After parturition, male rats offspring were
subjected for nociceptive behavioural testing (n=7 per group). In formalin test, their
nociceptive behavior score was recorded. The rats were sacrificed two hours post
formalin injection. After that, thalamus of the male rats offspring was removed to
determine the level of oxidative stress parameters and NMDA receptors while Nissl
staining was performed to detect the morphological changes in the thalamus. All data
were analysed by using SPSS, version 22. Nociceptive behaviour score was analysed
Description
Keywords
Honey , Toxicity