Dual Synthesisi Of Poly(3- Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer And Yello Pigment By Cupriavidus Sp. Usmahm13 And Its Characterization

Simple item page
dc.contributor.authorIsmail, Iszatty
dc.date.accessioned2019-08-29T01:17:22Z
dc.date.available2019-08-29T01:17:22Z
dc.date.issued2017-05
dc.description.abstractPoly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] copolymer has attracted intensive attention in various applications. Cupriavidus sp. USMAHM13 was found to have the ability of producing P(3HB-co-4HB) copolymer and yellow pigmentation simultaneously. Preliminary study had shown that the pigment extract exhibited antimicrobial activity against various bacteria. This present study was first conducted by applying various strategies to evaluate the effect of various carbon precursors on the biosynthesis of P(3HB-co-4HB) and yellow pigment. Combination of 1,4-butanediol with 1,6-hexanediol yielded a bacterial growth of 1.21 g/L to 6.72 g/L, PHA content of 28 wt% to 47 wt% and pigment concentration of 0.05 g/L to 0.18 g/L. Enhancement of P(3HB-co-4HB) copolymer and yellow pigment production were successfully achieved using 0.42 wt% C of 1,4-butanediol with 0.14 wt% C of 1,6- hexanediol at C/N=25 which resulted in an increased PHA content and pigment concentration of 52 wt% and 0.25 g/L, respectively. Further study on P(3HB-co-4HB) copolymer and yellow pigment production was also carried out through medium optimization. Addition of ammonium acetate was found to be the best nitrogen source in enhancing the P(3HB-co-4HB) accumulation. Prior to optimization of the yellow pigment production, screening for the best additional supplement was performed; resulting a high yield of yellow pigment when using yeast extract with optimal concentration of 2 g/L. Response surface methodology (RSM) was employed to evaluate and optimize the effect of different concentration of 1,4-butanediol, ammonium acetate and yeast extract.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8721
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectDual synthesisi of poly3- hydroxybutyrate-co-4-hydroxybutyrate copolymeren_US
dc.subjectYello pigment by cupriavidus sp. usmahm13en_US
dc.titleDual Synthesisi Of Poly(3- Hydroxybutyrate-Co-4-Hydroxybutyrate) Copolymer And Yello Pigment By Cupriavidus Sp. Usmahm13 And Its Characterizationen_US
dc.typeThesisen_US
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Pusat Pengajian Sains Kajihayat - Tesis