Comparison Of Protein Expression Between Breast And Colorectal Cancers
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Date
2012-03
Authors
Lee, Hui Hua
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Journal ISSN
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Publisher
Universiti Sains Malaysia
Abstract
Up to date, cancer remains a major health problem across the globe. Breast cancer is the most common cancer among women while colorectal cancer is the third most common cancer in both genders worldwide. In view of breast and colorectal cancer being among the most common types of cancer in Malaysia, we aimed to identify common biomarkers that can be used in the diagnosis or treatment of the cancers. This aim is accomplished by studying the protein expression profiles of breast and colorectal cancers tissues in comparison with their respective normal tissues. Twenty-five pairs of normal and cancerous tissues, respectively for breast and colorectal cancer were collected from Penang General Hospital (GH) with written informed consent obtained from the patients or their close relatives. Proteins were extracted from tissues by using sequential extraction method comprised of two extraction buffers, namely TRIS buffer and TLB buffer. The proteins were separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). By using the image analysis software, the proteome of each tissues type was compared between: i) breast cancerous and normal tissues; ii) colorectal cancerous and normal tissues and iii) breast and colorectal cancerous tissues. The differentially expressed protein spots were excised and subjected to LC-MS/MS analysis. In breast cancer, tropomyosin isoform was up-regulated in all studied N stages of all ethnics. Calrecticulin (TLB), PDI A3 (TLB), Hsp 70 (TLB), Hsp 60 and PDI were detected up-regulated in N0 and N1 stage in all the ethnic groups, but not in N2 stage. PCA and LDA statistic test revealed that the collectively used of PDI A3 (TRIS), activation-induced cytidine deaminase, alpha-1-antitrypsin precursor, alpha-1-antitrypsin, chain A, haptoglobin PDI, PDI A3 (TLB), annexin A3, annexin V, heat shock 70 kDa protein 1A, calrecticulin and tubulin alpha chain can reliably indicate pathological state of breast cancer. In colorectal cancer, annexin A3 was found consistently up-regulated in the entire N stages studied (namely N0, N1 and N2) and all ethnics. The PCA and LDA statistic test revealed that alpha-1-antitrypsin precursor, alpha-1-antitrypsin, chain A, PDI A3 (TRIS), Hsp 90 (TRIS), tubulin, beta polypeptide (TRIS), tubulin alpha chain A, PDI A3, tubulin, beta polypeptide (TLB), ATP synthase, Hsp 70 (TLB), annexin A3, and tropomyosin alpha 3 chain can be collectively used to differentiate normal and cancerous state of colorectal cancer.
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Keywords
Comparison of protein expression between , breast and colorectal cancers