Production of tannase by a local strain aspergillus niger FETL FT3 in submerged culture system.

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Date
2011-02
Authors
Ganeshan, Sumathi
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Publisher
Universiti Sains Malaysia
Abstract
Kultur kulat penghasil tanase yang dicamkan sebagai Aspergillus niger FETL FT3 telah dipencilkan dari tempatan daripada ekstrak tanin terhidrolisis kulit kayu bakau Rhizophora apiculata. Keupayaan kultur kulat tersebut untuk meningkatkan penghasilan tanase ekstrasel telah ditambahbaikan dalam sistem fermentasi kultur tenggelam (SmF). Penambahbaikan pelbagai proses parameter untuk penghasilan maksimum tanase ekstrasel oleh kultur kulat tersebut dilakukan dengan sel bebas dan sel tersekat-gerak di dalam kelalang goncangan 250 ml. A tannase-producing fungal culture identified as Aspergillus niger FETL FT3 was isolated locally from hydrolysed tannin extract of Rhizophora apiculata barks. The potential of the fungal culture for enhanced extracellular production of tannase was improved in submerged fermentation (SmF) system. The improvement of various process parameters for maximum production of extracellular tannase from the fungal culture was carried out with free and immobilized cells in 250 ml shake flasks.
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Keywords
Local strain , Culture system
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