Identification of copper-inducible genes in pistia stratiotes

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dc.contributor.authorMohd Azzam, Mohd Azad
dc.date.accessioned2015-07-30T05:29:05Z
dc.date.available2015-07-30T05:29:05Z
dc.date.issued2009
dc.description.abstractIn this study, a widely used plant to investigate freshwater pollution, Pistia stratiotes was utilized as a model system to determine the rate of copper uptake and to identify the candidate gene(s) that was specifically induced in response to copper challenge. The plant was exposed to various concentrations of copper (0, 1, 5, 10, 25, 50, 75, 100, 150 and 250 ~g/ml) for 24 hours. Generally, it was found that the amount of copper absorbed by Pistia stratiotes increased as the concentrations of the heavy metal exposed to the plant increased. Interestingly, the content of copper uptake by root was higher than to that of shoot. ACP-based RT-PCR method was used to identify copper-inducible genes. Three copperinducible candidate gene fragments were identified and were found to be upregulated in Pistia stratiotes exposed to copper in dose- and time-dependent manner. These findings strongly indicate that all three identified genes were positively induced in copper-treated Pistia stratiotes. Using RLM-RACE, a full length copper-inducible gene of fragment 1 was successfully cloned. Sequence analysis revealed that the full length gene shared 56% and 71% identity at nucleotide and amino acid levels, respectively, with Elaeis guineensis asparagine synthase-retated protein.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1052
dc.language.isoenen_US
dc.subjectCopper-inducible genesen_US
dc.subjectPistia stratiotesen_US
dc.titleIdentification of copper-inducible genes in pistia stratiotesen_US
dc.typeThesisen_US
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