Annual meeting of the Japanese pharmacological society
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Date
2012-03
Authors
Bakar, Ruzilawati Abu
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia
Abstract
High-performance liquid chromatography (HPLC) was
compared with gas chromatography-mass spectrometry
(GCMS) for determination of morphine in human urine.
Calibration standards were prepared In human urine.
Codeine and d3-morphine were added as the Internal
standards for HPLC and GCMS. respectively. After solidphase
extraction, the reconstituted samples were divided
rnto aliquots for analysis br HPLC and GCMS. The
analytical performances o the two methods were
compared with regard to sensitivity, precision and
accuracy. Results Indicated that tfPlC analysis
produced results at least as precise, accurate and
specific as GCMS for morphrne. Thus, analytical results
by HPLC were comparable to GCMS. Therefore. HPLC
can be useful alternative to GCMS for measuring
morphine in urine.
Description
Keywords
chromatography