The expression of insulin-like growth factors and their receptors at pre-implantation stage in reproductive tissue of diabetic mouse

Simple item page
dc.contributor.authorRahimah, Zakaria
dc.date.accessioned2014-11-11T08:30:23Z
dc.date.available2014-11-11T08:30:23Z
dc.date.issued2007
dc.descriptionPh.Den_US
dc.description.abstractWe hypothesized that the alteration in the expression of IGFs and their receptors may create an abnormal intrauterine environment thus affect embryos development. Therefore, the aims of the present study were to determine the effects of diabetes on in vitro development of mouse preimplantation embryos and to determine the mRNA and protein expression of IGF-1, IGF-2, IGF-1R and IGF-2R in the fallopian tube and uterine tissue of control and diabetic mice. Sexually mature female ICR mice of 6-8 weeks old were made diabetic by streptozotocin (200 mg/kg, intraperitoneal). The normal two-cell embryos were obtained from superovulated control and diabetic mice at 48 post-hCG treatment. Embryos were separately cultured and examined under an inverted microscope for 3 consecutive days. Fallopian tubes and uterine tissues were obtained from the superovulated control and diabetic mice at 48, 72 and 96 hours post-hCG treatment. The mRNA expression was measured using Realtime PCR using specifically developed homologous internal standards for each gene. Protein expression was measured by immunohistochemical staining and a semiquantitative scoring was performed using a standardized 5-scale system. The number of normal two-cell embryos obtained from diabetic mice was much reduced when compared to control mice. However, there was no significant difference in the percentage of two-cell embryo development in control and diabetic mice. The mRNA expression of IGF-1 in the fallopian tube and uterus of diabetic mice was significantly low at 72 hours and 96 hours post-hCG treatment, respectively. The mRNA expression of IGF-1R remained high in the fallopian tube but was significantly low in the uterus of diabetic mice at 96 hours post-hCG treatment. The mRNA expression IGF-2 in the fallopian tube was significantly high at 48 and 96 hours post-hCG treatment but was significantly low in the uterus of diabetic mice at 96 hours post-hCG treatment. The mRNA expression of IGF-2R in the fallopian tube and uterus of diabetic mice was significantly high at 48 and 96 hours, and at 48 hours post-hCG treatments, respectively. For protein expression, the immunohistochemical scoring for both IGF-1 and IGF-1R was significantly decreased in the fallopian tube of diabetic mice at 96 hours post-hCG treatment. In contrast, the score for IGF-2 and IGF- 2R was significantly increased in the fallopian tube of diabetic mice at 48 and 72 hours; and at 72 hours post-hCG treatment, respectively. However, there was no significant difference in the score of IGFs and their receptors in the uterus of control and diabetic mice. In conclusion, the percentage of the two-cell stage embryos which developed to blastocysts was similar in control and diabetic groups but whether the quality of these embryos were the same could not be confirmed. Both the mRNA and protein expression of IGFs and their receptors were significantly altered by maternal diabetes, which suggest their role in the pathogenesis of diabetic embryopathy.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/431
dc.language.isoenen_US
dc.subjectMedical scienceen_US
dc.subjectInsulin-like growth factorsen_US
dc.titleThe expression of insulin-like growth factors and their receptors at pre-implantation stage in reproductive tissue of diabetic mouseen_US
dc.typeThesisen_US
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Pusat Pengajian Sains Farmasi - Tesis