An Experimental Study On Antihypertensive Activity Of Garcinia Atroviridis Fruit Extracts
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Date
2014-01
Authors
Kharuddin, Fatim
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Garcinia atroviridis (GA) plant has been used as a traditional medicine for treating
earache, throat irritation, cough, dandruff and stomach disorders associated with
pregnancy and hypertension. In the present study, the anti-hypertensive effectsguided
extraction and fractionation of GA fruit were carried out in an attempt to find
the most active chemical component. The fruit of this plant was dried, ground and
serially extracted with petroleum ether, chloroform, methanol and water. The extracts
were dried under reduced pressure and later freeze-dried. The vasodilator effect of
the extracts was examined on isolated rat aortic ring preparation. The petroleum ether
extract was found to be the most potent in relaxing PE pre-contracted aortic rings.
The petroleum ether extract was then fractionated with n-hexane and
dichloromethane. The n-hexane fraction (2 mg ml-1) was found to be the most active
in relaxing the aortic ring. Removal of the endothelium of the aortic ring did not
abolish the relaxing property of petroleum ether extract which suggests that the
relaxing effect of the extract is not endothelium dependent. Orally administered all of
GA extracts (1 g kg-1) significantly reduced the systolic blood pressure and heart rate
of spontaneous hypertensive (SH) rats which support the antihypertensive effect of
GA fruit. In anaesthetized rats, the intravenous administration of methanol and water
extracts of GA dose-dependently reduced mean arterial pressure (MAP), systolic
pressure (SP), diastolic pressure (DP) and heart rate (HR) of SD rats.
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Keywords
Antihypertensive Activity , Garcinia Atroviridis Fruit Extracts