Microbial Degradation of Crude Oil by Acinetobacter baumannii T30c Isolated from Petroleum Hydrocarbon Contaminated Areas

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dc.contributor.authorChang, Lee Kok
dc.date.accessioned2018-08-02T07:21:43Z
dc.date.available2018-08-02T07:21:43Z
dc.date.issued2010-01
dc.description.abstractBioremediation technology comes of age due to frequent occurrence of oil pollution accidents which have caused ecological and social catastrophe. The aims of the project were to isolate the potential hydrocarbon-degrading bacteria and to study a biodegradation potential of the selected bacteria in a flask system, bioreactor system and application of bacterial inoculum in the Tapis crude oil-contaminated soil. One of the bacterial isolate, namely T30C was screened as potential hydrocarbons degrader. Bacterial isolate T30C was identified as Acinetobacter baumannii by morphology characterization, biochemical test, identification kit of RapiD™ NF Plus System (remel) and molecular level identification of 16S rRNA. Crude oil biodegradation study of Acinetobacter baumannii T30C with the following optimum condition of pH 8.0, 15 % (v/v) of inoculum size, temperature of 37 °C and agitation rate of 200 rpm resulted the maximum dry cell weight production of 0.12 g/L and percentage of crude oil degradation of 20 % of 1 % of crude oil addition after optimization of physical parameters. After optimization of chemical parameters on crude oil biodegradation study, the bacterial growth of 2.20 giL of dry cell weight and crude oil biodegradation of 67 % were obtained with the optimum medium composition as following: (NH4)2S04, 3 g; KH2P04, 4 g; Na2HP04, 7 g; MgS04·7HzO, 0.2 g; CaClz·2H20, 0.001 g; FeS04·7HzO, 0.001 g; Tween 80, 4.0 g/L in 1 L of distilled water (MSM) added with 1.0 %(w/v) of xxvii Tapis crude oil. By monitoring the different levels of physical parameters of agitation rate and flow rate in a stirred-tank bioreactor, maximum growth of 4.14 giL of dry cell weight and Tapis crude oil biodegradation of 67 % was achieved at the condition of 400 rpm and 0.67 vvm, with supplementation of 2.5 % (w/v) of crude oil. Finally, the sustainability growth of the bacteria isolate Acinetobacter baumannii T30C was tested, which bacteria was augmented in Tapis crude oil-contaminated soil based on the viable cell count and reduction of residual hydrocarbon extracted from the soil. Analysis of gas chromatography-flame ionization detection (GC-FID) showed that most aliphatic hydrocarbons (n-alkanes) were degraded extensively by bacterial isolate Acinetobacter baumannii T30C.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/6138
dc.subjectAcinetobacter baumanniien_US
dc.titleMicrobial Degradation of Crude Oil by Acinetobacter baumannii T30c Isolated from Petroleum Hydrocarbon Contaminated Areasen_US
dc.typeThesisen_US
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