Determination Of Selected Organochlorine Pesticides In Farmers’ Biological Samples Using Gas Chromatography-Mass Spectrometry And Their Knowledge, Attitude And Practice Towards Pesticides Use

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dc.contributor.authorNoor, Juliana Mohd
dc.date.accessioned2018-08-01T07:28:11Z
dc.date.available2018-08-01T07:28:11Z
dc.date.issued2011-03
dc.description.abstractThis study was conducted in agricultural areas in Pinang Tunggal, Kepala Batas, Penang. The main objective of the study was to determine the level of selected organochlorine pesticides in farmers’ biological samples particularly in hair and urine samples by using Gas Chromatography – Mass Spectrometry (GC-MS). Concomitantly, it was also carried out to assess the farmers` knowledge, attitude and practice towards pesticide use. Organochlorine pesticides selected in this study were HCHs (α-, β-, γ- and δ-hch), DDTs (p,p’-ddt, p,p’-ddd and p,p’-dde), aldrin, dieldrin, endosulfan sulfate, heptachlor, heptachlor epoxide and methoxychlor. Hair sample was chosen as it is known to be a good long term indicator for chemical exposure while urine is normally used to determine short term exposure. Method development and validation using GC-MS were established and several parameters were analyzed. Parameters observed were speed and time for agitation, speed and time for centrifugation, temperature for hair digestion and type of solvent for extraction. R2 was in accepted range, coefficients of variation were less than 20% and recoveries were in the range of 70% to 120%. A total 33 % of the hair samples (n=60) and 21% of the urine samples (n=60) from the respondents were detected to contain certain concentrations of the pesticides. β-HCH was the most organochlorine pesticide detected in hair, while Dieldrin was mostly found in urine. On weigh to weigh basis, higher concentration of organochlorine pesticides (OCPs) were detected in hair as compared to those found in urine. Others pesticides under study were not detected at all. They were 122 respondents participated in a self-administered survey on their knowledge, attitude and practice towards pesticides use. Based on the finding, most of the farmers seemed to have high knowledge towards pesticides use. High percentage (67 – 91%) of farmers appeared to possess reasonably good attitude in pesticide use based on their answers on the questions related to individual hygiene, how pesticides should be kept, whether pesticides containers can be re-used or not, and disposal of pesticides containers. More than 80% of respondents practiced the appropriate and safe way in handling and spraying pesticides. High level of knowledge towards pesticide use was noted. Very few claims of chronic effects on health were made. This study has successfully developed an assay for simultaneous quantification of selected organochlorine pesticides.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/6107
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectDetermine the level of selected organochlorine pesticides in farmersen_US
dc.subjectknowledge, attitude and practice towards pesticide useen_US
dc.titleDetermination Of Selected Organochlorine Pesticides In Farmers’ Biological Samples Using Gas Chromatography-Mass Spectrometry And Their Knowledge, Attitude And Practice Towards Pesticides Useen_US
dc.typeThesisen_US
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