Anti-Inflammatory And Analgesic Activities Of Garcinia Atroviridis Fruit Extracts
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Date
2014-01
Authors
Mansor, Farah Suhana
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Garcinia atroviridis (GA) is an edible medicinal herb traditionally used for the
treatment of earache, throat irritation, cough, dandruff, swollen abdomen and
postpartum medication. In the present study, the anti-inflammatory and analgesics
activity-guided extraction and fractionation of GA fruit were carried out in an
attempt to identify anti-inflammatory and analgesic agents which are more potent
and with less side effects. The GA fruits were sliced, dried, pulverized and serially
extracted with petroleum ether, chloroform, methanol and water using maceration
method. The extracts were dried under reduced pressure and freeze-dried. The
extraction yield were 1.45%, 0.89%, 21.24% and 7.99% petroleum ether (PE),
chloroform (CE), methanol (ME) and water (WE) extracts, respectively. The antiinflammatory
activity of the extracts was studied using carrageenan-induced hind
paw oedema in rat. Only administered ME at doses of 500 and 1000 mg/kg were
found significantly inhibited the hind paw oedema. ME was then fractionated into
chloroform (CF), ethyl acetate (EAF), n-butanol (nBF) and water (WF) fractions.
Among these four fractions, WF was found to be the most potent in inhibiting the
oedema formation. The anti-inflammatory activity of WF was further studied in
cotton pellet-induced granuloma in rats, a sub-chronic model of inflammation. WF
(125, 250 and 500 mg/kg) significantly inhibited the granuloma formation (p<0.001).
Blood cytokine analysis showed that the level of TNF-α and IL-1 in the WF orally
treated rats were significantly lower than the control group.
Description
Keywords
Anti-Inflammatory And Analgesic , Garcinia Atroviridis Fruit