An Analytical Evaluation Of In Vitro Drug-Drug Interaction Studies For Fixed Artesunate Combination Therapy

Simple item page
dc.contributor.authorDaniel Jayaraj, Vanessa Shalini
dc.date.accessioned2018-08-29T07:53:51Z
dc.date.available2018-08-29T07:53:51Z
dc.date.issued2010-04
dc.description.abstractDrug-resistant Plasmodium falciparum has become a serious threat, especially in malaria-endemic regions of the world. In light of this, the WHO has recommended artemisinin combination therapy such as artesunate-mefloquine and artesunate-amodiaquine as first line treatment for uncomplicated falciparum malaria especially in regions harbouring the most resistant isolates. However, the in vitro drug interaction studies have not been conducted for both these combinations. An in vitro experimental technique, utilizing human liver microsomes was used to study the metabolism of AS in the presence and absence of mefloquine and amodiaquine. Simultaneous determination of artesunate, dihydroartemisinin and artemisinin in microsomal sample was performed using high performance liquid chromatography- electrochemical (HPLC-EC) method. The study shows that artesunate is metabolised to dihydroartemisinin in the in vitro microsomal assay. The reaction is mediated by CYP450 enzymes present in the microsomal assay. The metabolism of artesunate was not affected by mefloquine or amodiaquine at therapeutic concentrations using the in vitro microsomal technique. The absence of interactions between these drugs led to the conclusion that the artesunate-mefloquine and artesunate-amodiaquine combinations are ideal and safe treatments when used in therapeutic concentrations. However, further clinical studies must be done to confirm these findings.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/6466
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectAn analytical evaluation of in vitro drug-drug interactionen_US
dc.subjectfor fixed artesunate combination therapyen_US
dc.titleAn Analytical Evaluation Of In Vitro Drug-Drug Interaction Studies For Fixed Artesunate Combination Therapyen_US
dc.typeThesisen_US
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Pusat Penyelidikan Dadah dan Ubat-Ubatan - Tesis