Immobilization of β-galactosidase onto activated chitosan support by using covalent binding method
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Date
2018-06
Authors
Nur Syahirah Sezale
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Abstract
The performance of immobilized β-galactosidase (β-Gal) onto chitosan
support was studied in a batch process. The chitosan support was activated by using
chemical agent which was glutaraldehyde solution. The main objective of this research
was to investigate the suitable conditions in preparation of β-Gal immobilization
process that will produce greater β-Gal activity. During the preparation of immobilized
β-Gal, the optimum conditions were obtained from Response Surface Methodology
(RSM). The optimum conditions were found to be 2% and 10 mg/ml for glutaraldehyde
and β-Gal concentrations, respectively, which has resulted in 65.52% for activity yield
meanwhile for immobilization yield, is 94.57%. The experimental results for
investigation of effect of temperature on β-Gal activity, found that the optimum
temperature at ℃ was obtained after β-Gal immobilization. However, it is so
interesting that the immobilized β-Gal showed much better operational stability
referring to a broader thermal range as compared to free β-Gal. The activity of
immobilized β-Gal increased at about more than 17% of its initial activity, meanwhile
the activity of free β-Gal reduced at less than 15%. Higher stability of β-Gal will lead to
a better reusability. The immobilized β-Gal can be reused for six cycles and retained
approximately 50% of its initial activity.