Immobilization of β-galactosidase onto activated chitosan support by using covalent binding method
| dc.contributor.author | Nur Syahirah Sezale | |
| dc.date.accessioned | 2021-04-22T05:32:10Z | |
| dc.date.available | 2021-04-22T05:32:10Z | |
| dc.date.issued | 2018-06 | |
| dc.description.abstract | The performance of immobilized β-galactosidase (β-Gal) onto chitosan support was studied in a batch process. The chitosan support was activated by using chemical agent which was glutaraldehyde solution. The main objective of this research was to investigate the suitable conditions in preparation of β-Gal immobilization process that will produce greater β-Gal activity. During the preparation of immobilized β-Gal, the optimum conditions were obtained from Response Surface Methodology (RSM). The optimum conditions were found to be 2% and 10 mg/ml for glutaraldehyde and β-Gal concentrations, respectively, which has resulted in 65.52% for activity yield meanwhile for immobilization yield, is 94.57%. The experimental results for investigation of effect of temperature on β-Gal activity, found that the optimum temperature at ℃ was obtained after β-Gal immobilization. However, it is so interesting that the immobilized β-Gal showed much better operational stability referring to a broader thermal range as compared to free β-Gal. The activity of immobilized β-Gal increased at about more than 17% of its initial activity, meanwhile the activity of free β-Gal reduced at less than 15%. Higher stability of β-Gal will lead to a better reusability. The immobilized β-Gal can be reused for six cycles and retained approximately 50% of its initial activity. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/13071 | |
| dc.language.iso | en | en_US |
| dc.title | Immobilization of β-galactosidase onto activated chitosan support by using covalent binding method | en_US |
| dc.type | Other | en_US |
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