The Roles Of Ferric Uptake Regulator (Fur) For Plant Root Attachment By Burkholderia sp. USMB20

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dc.contributor.authorShaffie, Salwani
dc.date.accessioned2019-04-25T06:43:17Z
dc.date.available2019-04-25T06:43:17Z
dc.date.issued2018-05
dc.description.abstractAttachment is an early step during bacterial plant root colonization process. Bacteria need iron as an essential component for its growth and cellular processes. Ferric uptake regulator (Fur) is a global regulator that controls bacterial iron uptake. This study investigated the roles of Burkholderia sp. USMB20’s Fur in controlling iron uptake during plant root attachment. USMB20 Fur minus mutant (USMB20Efur) was used as comparison. The observation showed USMB20 recorded highest number of attached cell on root surface in 50μM of ferric concentration, where as USMB20Efur recorded less numbers of attached cell. Scanning Electron Microscope (SEM) showed dense attachment of cells on the root surface compared to the USMB20Efur. This study has demonstrated that Fur influenced several mechanisms that enhanced bacterial attachment on root. Analysis of protein using Two Dimension Electrophoresis (2DE) and peptide sequencing revealed that Fur controlled the up regulation of several USMB20 proteins during USMB20 attachment nevertheless down regulation for USMB20Efur. These proteins were identified as molecular chaperone groEL, elongation factor TU, xylose isomerase and alkyl hydrogenase peroxide. Based on relative qualitative gene expression via qPCR, genes (groEL, tuf, xylA and ahpC) coding for these Fur-regulated proteins were discovered to be induced in USMB20 during root attachment, while no gene expression was observed in USMB20Efur. Additionally, each up regulated gene had putative Fur-box consensus sequence. The Fur would recognize this region prior to initiation of the transcription of these genes. In summary, Fur is important in controlling iron uptake and regulating several essential proteins during plant root attachment by USMB20en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8113
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectThe roles of ferric uptake regulatoren_US
dc.subjectfor plant root attachmenten_US
dc.titleThe Roles Of Ferric Uptake Regulator (Fur) For Plant Root Attachment By Burkholderia sp. USMB20en_US
dc.typeThesisen_US
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