Study Of Pithecellobium Jiringa (Jack) Prain On Carcinogenesis And Angiogenesis Mechanistic Pathway

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dc.contributor.authorMuslim, Nahdzatul Syima
dc.date.accessioned2019-06-25T07:31:53Z
dc.date.available2019-06-25T07:31:53Z
dc.date.issued2012-06
dc.description.abstractIn this work, the anti-tumour potential of P. jiringa fruit rind was investigated. An array of studies was carried out to evaluate its anti-tumour effect and characterised the components at play and the biological target(s) that contribute to the anti-tumour effect. In the first part of this work, a series of solvent extract(s) of the P. jiringa fruit rind namely 100% ethanol (EtOH), 50% ethanol (EW), 50% methanol (MW), 100% methanol (MeOH) and 100% water (Wa) was developed via maceration technique. The extracts gave strong cytostatic response on human umbilical vein endothelial cells (HUVEC) (IC50 0.60 ± 0.15 μg/ml and 3.01± 0.05 μg/ml for EtOH and EW respectively) and strong selective cytotoxic activity on colorectal cancer cells (HCT 116) (IC50 5.54 ± 0.13 μg/ml and 12.63 ± 0.17 μg/ml for EtOH and EW respectively) The in vitro anti-cancer study on HCT 116 cells showed that both EtOH and EW extracts caused cell death via extrinsic pathway. In the HUVEC cells we found that both EW and EtOH disrupted cell migration and endothelial tube formation. The extracts caused marked inhibition of angiogenesis in isolated tissue assay and inhibited the expression of VEGF which is the key marker of angiogenesis. The anti-angiogenic activity was also observed in vivo in fertilised chicken egg embryo. Both EW and EtOH showed abundance of phenolic compounds giving rise to strong antioxidant property. The latter may be the key factor that contributes to the anti-angiogenic activity observed. The result of this study suggests that EW and EtOH extracts may be useful in cancer therapy particularly towards the highly angiogenic dependent tumours such as colorectal cancer.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8381
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectStudy of Pithecellobium jiringa (Jack) prainen_US
dc.subjectcarcinogenesis and angiogenesis mechanistic pathwayen_US
dc.titleStudy Of Pithecellobium Jiringa (Jack) Prain On Carcinogenesis And Angiogenesis Mechanistic Pathwayen_US
dc.typeThesisen_US
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