ANALISIS GENETIK MOLEKUL DAN IMUNOFENOTIP PENYAKIT NON-HODGKIN'S LYMPHOMA DI KELANTAN
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Date
2001-12
Authors
KHAIRANI, IDAH MOKHTAR
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Perubatan Universiti Sains Malaysia
Abstract
Non-Hodgkin's lymphoma (NHL) is a heterogeneous malignancy. Recent advancement in
techniques in the field of immunology and molecular biology enables the diagnosis and
classifications of NHL to be made satisfactorily. Protein expression criteria such as p53
and bcl-2 play an important role in classifying lymphoma. There are three main objectives
in this study. First, to observe the effectiveness of flow cytometry analysis as a technique
for lineage assignment in NHL using fresh single cell suspension samples. Second, to
detect immunoglobulin heavy (lgH) chain gene rearrangement as a technique to identify
monoclonal population in NHL cases by polymerase chain reaction (PCR). Third, to detect
. .
the expression and distribution of p53 and bcl-2 protein on different grades of NHL by
immunohistochemistry technique.
Lymph node samples were obtained from patients suspected of having lymphoma. In flow
cytometry analysis, single cell suspension were prepared from 37 lymph node samples,
reacted with monoclonal antibodies and analysed using FACScan machine. Detection of
IgH gene rearrangement was done using DNA extracted from 43 fresh or frozen lymph
nodes and was subjected to PCR using VH/JH specific primer. Monoclonal population was
represented by amplified band at 90-160bp. Immunohistochemistry staining was done
using monoclonal antibodies p53 (00-7) and bcl-2 (clone 124) on 56 paraffin embedded
tissue sections.
Description
Keywords
Non-Hodgkin's lymphoma